Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6257] to NAPSIN A
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-NAPSIN A antibody [EPR6257]
See all NAPSIN A primary antibodies
DescriptionRabbit monoclonal [EPR6257] to NAPSIN A
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Synthetic peptide within Human NAPSIN A aa 50-150. The exact sequence is proprietary.
- WB: Human lung adenocarcinoma lysate. IHC-P: Human lung tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 6.17 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab129189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMay be involved in processing of pneumocyte surfactant precursors.
Tissue specificityExpressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Sequence similaritiesBelongs to the peptidase A1 family.
- Information by UniProt
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with purified ab129189 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/5000 dilution (purified) + Human lung adenocarcinoma tissue lysate at 20 µg
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM /TBST.
Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/1000 dilution (unpurified) + Human lung adenocarcinoma lysate at 10 µg
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with unpurified ab129189 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129189 has not yet been referenced specifically in any publications.