RecombinantRabMAb

Recombinant Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
  • Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
  • Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
  • OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
  • Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6257] to NAPSIN A - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free
    See all NAPSIN A primary antibodies

  • Description

    Rabbit monoclonal [EPR6257] to NAPSIN A - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248340 is the carrier-free version of ab129189 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      May be involved in processing of pneumocyte surfactant precursors.

    • Tissue specificity

      Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.

    • Sequence similarities

      Belongs to the peptidase A1 family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Asp 4 antibody
        • ASP4 antibody
        • Aspartyl protease 4 antibody
        • KAP antibody
        • Kdap antibody
        • Kidney derived aspartic protease like protein antibody
        • NAP1 antibody
        • NAPA antibody
        • Napsa antibody
        • NAPSA_HUMAN antibody
        • Napsin 1 antibody
        • napsin A aspartic peptidase antibody
        • Napsin A precursor antibody
        • Napsin-1 antibody
        • Napsin-A antibody
        • Pronapsin A antibody
        • SNAPA antibody
        • TA01/TA02 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        This data was developed using ab129189, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with purified ab129189 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
      • Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/5000 dilution (purified) + Human lung adenocarcinoma tissue lysate at 20 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

        Predicted band size: 45 kDa
        Observed band size: 50 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab129189, the same antibody clone in a different buffer formulation.

        Blocking and dilution buffer: 5% NFDM/TBST.

      • Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Western blot - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/1000 dilution (unpurified) + Human lung adenocarcinoma lysate at 10 µg

        Secondary
        HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 45 kDa



        This data was developed using ab129189, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        This data was developed using ab129189, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with unpurified ab129189 at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
      • OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        OI-RD Scanning - Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        This data was developed using ab129189, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)
        Anti-NAPSIN A antibody [EPR6257] - BSA and Azide free (ab248340)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248340? Please let us know so that we can cite the reference in this datasheet.

      ab248340 has not yet been referenced specifically in any publications.

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