Anti-NAPSIN A antibody [KCG1.1] (ab73021)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-NAPSIN A antibody [KCG1.1]
See all NAPSIN A primary antibodies -
Description
Mouse monoclonal [KCG1.1] to NAPSIN A -
Host species
Mouse -
Specificity
Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive. -
Tested applications
Suitable for: Flow Cyt, ICC, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide from the N terminus of human NAPSIN A
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Positive control
- Lung Adenocarcinoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
KCG1.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab73021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC | 1/100. | |
| ICC/IF | 1/50. (customer data using A549 cells) | |
| IHC-P | 1/50 - 1/100. 30 minutes at room temperature in an ABC method. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [KCG1.1] (ab73021)](https://www.abcam.com/ps/products/73/ab73021/Images/ab73021-88245-anti-napsin-a-antibody-kcg11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [KCG1.1] (ab73021)ab73021, at 1 in 50 dilution, staining NAPSIN A in Human lung adenocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. -
![Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)](https://www.abcam.com/ps/products/73/ab73021/Images/ab73021-88244-anti-napsin-a-antibody-kcg11-flow-cytometry.jpg)
Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)](https://www.abcam.com/ps/products/73/ab73021/Images/ab73021-88243-anti-napsin-a-antibody-kcg11-immunocytochemistry.jpg)
Immunocytochemistry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)Image courtesy of an anonymous Abreview.ab73021 staining NAPSIN A in cells from human broncho-alveolar lavage by Immunocytochemistry.
Cells were fixed in formaldehyde, permeabilized using Triton, blocked with 5% normal horse serum for 1 hour at room temperature and then incubated with ab73021 at a 1/100 dilution for 16 hours. The secondary used was a biotin conjugated horse anti-mouse polyclonal used at a 1/300 dilution.
Protocols
References
This product has been referenced in:
- Tian HW et al. Multiple pulmonary metastases with halo-sign from malignant mixed Müllerian tumors. Oncol Lett 14:6645-6649 (2017). Read more (PubMed: 29151911) »
- Zhao J et al. CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients. Cell Physiol Biochem 44:2138-2146 (2017). Read more (PubMed: 29241190) »
