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    napsin-a-antibody-kcg11-ab73021.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-NAPSIN A antibody [KCG1.1] (ab73021)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (3)

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Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)

    Key features and details

    • Mouse monoclonal [KCG1.1] to NAPSIN A
    • Suitable for: Flow Cyt, IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    Overview

    • Product name

      Anti-NAPSIN A antibody [KCG1.1]
      See all NAPSIN A primary antibodies
    • Description

      Mouse monoclonal [KCG1.1] to NAPSIN A
    • Host species

      Mouse
    • Specificity

      Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive.
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide from the N terminus of human NAPSIN A

    • Positive control

      • IHC-P: Lung Adenocarcinoma tissue. Flow cyt: A549 cells.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituents: 1% BSA, PBS
    • Concentration information loading...
    • Purity

      IgG fraction
    • Clonality

      Monoclonal
    • Clone number

      KCG1.1
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Other proteases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human NAPSIN A protein (denatured) (ab202144)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab73021 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    Application Abreviews Notes
    Flow Cyt
    1/50 - 1/100.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    IHC-P
    1/50 - 1/100. 30 minutes at room temperature in an ABC method. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining
    Notes
    Flow Cyt
    1/50 - 1/100.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    IHC-P
    1/50 - 1/100. 30 minutes at room temperature in an ABC method. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining

    Target

    • Function

      May be involved in processing of pneumocyte surfactant precursors.
    • Tissue specificity

      Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession O96009 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9476 Human
      • Omim: 605631 Human
      • SwissProt: O96009 Human
      • Unigene: 512843 Human
      • Alternative names

        • Asp 4 antibody
        • ASP4 antibody
        • Aspartyl protease 4 antibody
        • KAP antibody
        • Kdap antibody
        • Kidney derived aspartic protease like protein antibody
        • NAP1 antibody
        • NAPA antibody
        • Napsa antibody
        • NAPSA_HUMAN antibody
        • Napsin 1 antibody
        • napsin A aspartic peptidase antibody
        • Napsin A precursor antibody
        • Napsin-1 antibody
        • Napsin-A antibody
        • Pronapsin A antibody
        • SNAPA antibody
        • TA01/TA02 antibody
        see all

      Images

      • Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)
        Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)
        Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab73021? Please let us know so that we can cite the reference in this datasheet.

      ab73021 has been referenced in 3 publications.

      • Guan S  et al. TGF-ß1 induces CREB1-mediated miR-1290 upregulation to antagonize lung fibrosis via Napsin A. Int J Mol Med 46:141-148 (2020). PubMed: 32319530
      • Tian HW  et al. Multiple pulmonary metastases with halo-sign from malignant mixed Müllerian tumors. Oncol Lett 14:6645-6649 (2017). PubMed: 29151911
      • Zhao J  et al. CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients. Cell Physiol Biochem 44:2138-2146 (2017). PubMed: 29241190

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Question

      Product code: 73021, Lot number: GR3195479-3
      What is the IgG concentration?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 14 2018

      Answer

      The IgG concnetration for batch GR3195479-3 is 20 ug/ml.

      Read More

      Abcam Scientific Support

      Answered on Jun 14 2018

      Immunocytochemistry abreview for Anti-NAPSIN A antibody [KCG1.1]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cell (cells from broncho-alveolar lavage)
      Specification
      cells from broncho-alveolar lavage
      Fixative
      Formaldehyde
      Permeabilization
      Yes - PBS triton 0.25% 10 min
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 16 2012

      Question

      Concentration of ab73021 batch GR4651

      Read More

      Abcam community

      Verified customer

      Asked on Dec 14 2011

      Answer

      Merci pour votre appel. Je viens de trouver l'information. Je peux donc confirmer que le ab73021 lot GR4651 est à une concentration de 100 ug/ml. Je vous souhaite une bonne soirée et bon succès pour l'expérience de demain!  

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2011

      Question

      ab73021 to be tested in ICC.  

      Read More

      Abcam community

      Verified customer

      Asked on Nov 29 2011

      Answer

      Code de remise : **** Date d’expiration : JJ MM AAAA Merci de nous avoir contactés de nouveau. Ma collègue du service clientèle m'a informé que vous aviez décidé de participer à notre offre et tester ab73021 en ICC. Le code de réduction correspondant est ****. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire. Vous devez dans un premier temps soumettre une Abreview avec vos résultats en ICC en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab73021. Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches. Termes et Conditions : www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Nov 29 2011

      Question

      ab73021 to be tested in ICC.  

      Read More

      Abcam community

      Verified customer

      Asked on Nov 25 2011

      Answer

      Merci de votre intérêt pour ab73021. A ma connaissance, cet anticorps n’a pas encore été testé en ICC et nous n’offrons pas d’échantillon gratuit à des fins de tests préliminaires. Cependant, si vous souhaitez tester ab73021 en ICC, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, vous pourrez recevoir un anticorps primaire gratuit lors d’une prochaine commande. Etapes à suivre : 1. Nous confirmer que vous souhaiteriez tester ab73021 en ICC afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab73021 2. Acheter ab73021 par téléphone, fax ou internet (www.abcam.com) sans indiquer le code promotionnel 3. Le tester en ICC 4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview et indiquer le code promotionnel. Pour plus d’informations https://www.abcam.com/abreviews 5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire. Même si ab73021 ne fonctionne pas en ICC, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps. N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle. Termes et Conditions : www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Nov 25 2011

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