Anti-NAPSIN A antibody [KCG1.1] (ab73021)
![Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)](https://www.abcam.com/ps/products/73/ab73021/Images/ab73021-88244-anti-napsin-a-antibody-kcg11-flow-cytometry.jpg "Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)")
Key features and details
- Mouse monoclonal [KCG1.1] to NAPSIN A
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NAPSIN A antibody [KCG1.1]
See all NAPSIN A primary antibodies -
Description
Mouse monoclonal [KCG1.1] to NAPSIN A -
Host species
Mouse -
Specificity
Napsin A reactivity was determined immunohistochemically on formalin-fixed paraffin-embedded cell blocks from 50 pleural effusion (5 mesotheliomas, 10 mesothelial proliferations, 12 pulmonary, and 23 nonpulmonary metastases). Napsin A was positive in 10/12 pulmonary adenocarcinomas. The specificity of this antibody for primary lung adenocarcinoma is 47 out of 58 (81%), primary lesions were positive. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
Synthetic peptide from the N terminus of human NAPSIN A
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Positive control
- IHC-P: Lung Adenocarcinoma tissue. Flow cyt: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
KCG1.1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
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| IHC-P |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50 - 1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P |
1/50 - 1/100. 30 minutes at room temperature in an ABC method.
Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining
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| Notes |
|---|
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Flow Cyt
1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
IHC-P
1/50 - 1/100. 30 minutes at room temperature in an ABC method. Formalin-fixed paraffin-embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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Flow Cytometry - Anti-NAPSIN A antibody [KCG1.1] (ab73021)Overlay histogram showing A549 cells stained with ab73021 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab73021, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab73021 has been referenced in 3 publications.
- Guan S et al. TGF-ß1 induces CREB1-mediated miR-1290 upregulation to antagonize lung fibrosis via Napsin A. Int J Mol Med 46:141-148 (2020). PubMed: 32319530
- Tian HW et al. Multiple pulmonary metastases with halo-sign from malignant mixed Müllerian tumors. Oncol Lett 14:6645-6649 (2017). PubMed: 29151911
- Zhao J et al. CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients. Cell Physiol Biochem 44:2138-2146 (2017). PubMed: 29241190