Anti-NAPSIN A antibody [NAPSA/1238] (ab234002)
Key features and details
- Mouse monoclonal [NAPSA/1238] to NAPSIN A
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NAPSIN A antibody [NAPSA/1238]
See all NAPSIN A primary antibodies -
Description
Mouse monoclonal [NAPSA/1238] to NAPSIN A -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human NAPSIN A aa 189-299. The exact sequence is proprietary.
Database link: O96009 -
Positive control
- WB: K562, HEK-293 and A549 cell lysates. IHC-P: Human lung adenocarcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from rabbit anti-serum by Protein A. -
Clonality
Monoclonal -
Clone number
NAPSA/1238 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.
(Primary incubation for 60 minutes at RT). |
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
Notes |
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Protein Array
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. (Primary incubation for 60 minutes at RT). |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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ab234002 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
All lanes : Anti-NAPSIN A antibody [NAPSA/1238] (ab234002) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : A549 (human chronic lung carcinoma cell line) cell lysate
Predicted band size: 45 kDa -
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for NAPSIN A using ab234002 at 2 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234002 has not yet been referenced specifically in any publications.