Key features and details
- Mouse monoclonal [NAPSA/1238] to NAPSIN A
- Suitable for: Protein Array, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-NAPSIN A antibody [NAPSA/1238]
See all NAPSIN A primary antibodies
DescriptionMouse monoclonal [NAPSA/1238] to NAPSIN A
Tested applicationsSuitable for: Protein Array, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NAPSIN A aa 189-299. The exact sequence is proprietary.
Database link: O96009
- WB: K562, HEK-293 and A549 cell lysates. IHC-P: Human lung adenocarcinoma tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified from rabbit anti-serum by Protein A.
Light chain typekappa
Our Abpromise guarantee covers the use of ab234002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Protein Array||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.
(Primary incubation for 60 minutes at RT).
|IHC-P||Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT).
FunctionMay be involved in processing of pneumocyte surfactant precursors.
Tissue specificityExpressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Sequence similaritiesBelongs to the peptidase A1 family.
- Information by UniProt
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
ab234002 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
All lanes : Anti-NAPSIN A antibody [NAPSA/1238] (ab234002) at 2 µg/ml
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : A549 (human chronic lung carcinoma cell line) cell lysate
Predicted band size: 45 kDa
Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for NAPSIN A using ab234002 at 2 µg/ml in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234002 has not yet been referenced specifically in any publications.