Overview

  • Product name

    Anti-NAPSIN A antibody [NAPSA/1238]
    See all NAPSIN A primary antibodies
  • Description

    Mouse monoclonal [NAPSA/1238] to NAPSIN A
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NAPSIN A aa 189-299. The exact sequence is proprietary.
    Database link: O96009

  • Positive control

    • WB: K562, HEK-293 and A549 cell lysates. IHC-P: Human lung adenocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab234002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.

(Primary incubation for 60 minutes at RT).

IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 minutes at RT).

Target

  • Function

    May be involved in processing of pneumocyte surfactant precursors.
  • Tissue specificity

    Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Asp 4 antibody
    • ASP4 antibody
    • Aspartyl protease 4 antibody
    • KAP antibody
    • Kdap antibody
    • Kidney derived aspartic protease like protein antibody
    • NAP1 antibody
    • NAPA antibody
    • Napsa antibody
    • NAPSA_HUMAN antibody
    • Napsin 1 antibody
    • napsin A aspartic peptidase antibody
    • Napsin A precursor antibody
    • Napsin-1 antibody
    • Napsin-A antibody
    • Pronapsin A antibody
    • SNAPA antibody
    • TA01/TA02 antibody
    see all

Images

  • ab234002 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • All lanes : Anti-NAPSIN A antibody [NAPSA/1238] (ab234002) at 2 µg/ml

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 3 : A549 (human chronic lung carcinoma cell line) cell lysate

    Predicted band size: 45 kDa

  • Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for NAPSIN A using ab234002 at 2 µg/ml in immunohistochemical analysis.

References

ab234002 has not yet been referenced specifically in any publications.

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