Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
![Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)](https://www.abcam.com/ps/products/213/ab213060/Images/ab213060-336745-anti-napsin-a-antibody-napsa-1238-protein-array.jpg "Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)")
Key features and details
- Mouse monoclonal [NAPSA/1238] to NAPSIN A - BSA and Azide free
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free
See all NAPSIN A primary antibodies -
Description
Mouse monoclonal [NAPSA/1238] to NAPSIN A - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment.
Database link: O96009 -
Positive control
- K562 and HEK293 cell lysates; Human lung adenocarcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab213060 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
NAPSA/1238 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab213060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (for 30 minutes at RT) |
|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. (for 60 minutes at RT) |
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| Protein Array | Use at an assay dependent concentration. |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)This data was produced with ab234002, the same antibody in a different formulation with BSA and Azide.
ab234002 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)](https://www.abcam.com/ps/products/213/ab213060/Images/ab213060-264432-anti-napsin-a-antibody-napsa-1238-western-blot.jpg)
Western blot - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : HEK293 cell lysate
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)](https://www.abcam.com/ps/products/213/ab213060/Images/ab213060-264433-anti-napsin-a-antibody-napsa-1238-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocaracinoma tissue labeling NAPSIN A with ab213060 at 2 µg/ml.
Protocols
Datasheets and documents
References (0)
ab213060 has not yet been referenced specifically in any publications.
