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Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)

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Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
  • Western blot - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)

Key features and details

  • Mouse monoclonal [NAPSA/1238] to NAPSIN A - BSA and Azide free
  • Suitable for: IHC-P, WB, Protein Array
  • Reacts with: Human
  • Isotype: IgG1

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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free
    See all NAPSIN A primary antibodies
  • Description

    Mouse monoclonal [NAPSA/1238] to NAPSIN A - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WB, Protein Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment.
    Database link: O96009

  • Positive control

    • K562 and HEK293 cell lysates; Human lung adenocarcinoma tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    ab213060 is purified from Bioreactor Concentrate by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    NAPSA/1238
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Associated products

  • Alternative Versions

    • Anti-NAPSIN A antibody [NAPSA/1238] (ab234002)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • HEK293 whole cell lysate (ab7902)
    • K562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human NAPSIN A protein (denatured) (ab202144)
  • Related Products

    • Recombinant Human NAPSIN A protein (denatured) (ab202144)

Applications

Our Abpromise guarantee covers the use of ab213060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(for 30 minutes at RT)

WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.

(for 60 minutes at RT)

Protein Array Use at an assay dependent concentration.

Target

  • Function

    May be involved in processing of pneumocyte surfactant precursors.
  • Tissue specificity

    Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession O96009 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9476 Human
    • Omim: 605631 Human
    • SwissProt: O96009 Human
    • Unigene: 512843 Human
    • Alternative names

      • Asp 4 antibody
      • ASP4 antibody
      • Aspartyl protease 4 antibody
      • KAP antibody
      • Kdap antibody
      • Kidney derived aspartic protease like protein antibody
      • NAP1 antibody
      • NAPA antibody
      • Napsa antibody
      • NAPSA_HUMAN antibody
      • Napsin 1 antibody
      • napsin A aspartic peptidase antibody
      • Napsin A precursor antibody
      • Napsin-1 antibody
      • Napsin-A antibody
      • Pronapsin A antibody
      • SNAPA antibody
      • TA01/TA02 antibody
      see all

    Images

    • Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
      Protein Array - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
      This data was produced with ab234002, the same antibody in a different formulation with BSA and Azide.
      ab234002 was tested in protein array against over 19000 different full-length human proteins.
      Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
      A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
    • Western blot - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
      Western blot - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
      All lanes : Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (ab212060) at 2 µg/ml

      Lane 1 : K562 cell lysate
      Lane 2 : HEK293 cell lysate

      Predicted band size: 45 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [NAPSA/1238] - BSA and Azide free (ab213060)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung adenocaracinoma tissue labeling NAPSIN A with ab213060 at 2 µg/ml.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab213060? Please let us know so that we can cite the reference in this datasheet.

    ab213060 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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