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    nardilysin-antibody-ab154237.pdf

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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Metalloprotease MMPs
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Anti-Nardilysin antibody (ab154237)

  • Datasheet
  • SDS
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Western blot - Anti-Nardilysin antibody (ab154237)
  • Western blot - Anti-Nardilysin antibody (ab154237)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nardilysin antibody (ab154237)

Key features and details

  • Rabbit polyclonal to Nardilysin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Nardilysin antibody
    See all Nardilysin primary antibodies
  • Description

    Rabbit polyclonal to Nardilysin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 543-787 of Human Nardilysin.

  • Positive control

    • NT2D1 and Mouse kidney whole cell lysates; HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • MMPs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse kidney normal tissue lysate - total protein (ab29305)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 132 kDa.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 132 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Relevance

    A member of the metalloproteinase family M16, nardilysin is most similar to insulin degrading enzyme, and the bacterial peptidase pitrilysin. It cleaves peptide substrates on the N terminus of arginine residues in dibasic pairs. Nardilysin was first described as a processing enzyme of somatostatin 1-28, which is cleaved at the Arg-Lys paired amino acid location. Nardilysin was named N arginine dibasic convertase (NRD convertase) to reflect this cleavage, although it was later shown that the activity was due to aminopeptidase B acting as a heterodimer with nardilysin. In addition to somatostatin 1-28, nardilysin cleaves dynorphin A and a-neoendorphin. Nardilysin was first detected in the brain and testis, and later in the heart, skeletal muscle, and in lesser amounts in most tissues and cell lines.
  • Database links

    • Entrez Gene: 4898 Human
    • Entrez Gene: 230598 Mouse
    • Omim: 602651 Human
    • SwissProt: O43847 Human
    • SwissProt: Q8BHG1 Mouse
    • Alternative names

      • EC 3.4.24.61 antibody
      • hNRD1 antibody
      • hNRD2 antibody
      • N arginine dibasic convertase antibody
      • Nardilysin precursor antibody
      • NRD C antibody
      • NRD convertase antibody
      • NRD1 antibody
      see all

    Images

    • Western blot - Anti-Nardilysin antibody (ab154237)
      Western blot - Anti-Nardilysin antibody (ab154237)
      Anti-Nardilysin antibody (ab154237) at 1/2000 dilution + NT2D1 whole cell lysate at 30 µg

      Predicted band size: 132 kDa



      5% SDS PAGE
    • Western blot - Anti-Nardilysin antibody (ab154237)
      Western blot - Anti-Nardilysin antibody (ab154237)
      Anti-Nardilysin antibody (ab154237) at 1/1000 dilution + Mouse kidney whole cell lysate at 50 µg

      Predicted band size: 132 kDa



      5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Nardilysin antibody (ab154237)
      Immunocytochemistry/ Immunofluorescence - Anti-Nardilysin antibody (ab154237)
      Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nardilysin with ab154237 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab154237? Please let us know so that we can cite the reference in this datasheet.

    ab154237 has not yet been referenced specifically in any publications.

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