Anti-Nardilysin antibody (ab154237)
Key features and details
- Rabbit polyclonal to Nardilysin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nardilysin antibody
See all Nardilysin primary antibodies -
Description
Rabbit polyclonal to Nardilysin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within internal sequence amino acids 543-787 of Human Nardilysin.
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Positive control
- NT2D1 and Mouse kidney whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 132 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 132 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
A member of the metalloproteinase family M16, nardilysin is most similar to insulin degrading enzyme, and the bacterial peptidase pitrilysin. It cleaves peptide substrates on the N terminus of arginine residues in dibasic pairs. Nardilysin was first described as a processing enzyme of somatostatin 1-28, which is cleaved at the Arg-Lys paired amino acid location. Nardilysin was named N arginine dibasic convertase (NRD convertase) to reflect this cleavage, although it was later shown that the activity was due to aminopeptidase B acting as a heterodimer with nardilysin. In addition to somatostatin 1-28, nardilysin cleaves dynorphin A and a-neoendorphin. Nardilysin was first detected in the brain and testis, and later in the heart, skeletal muscle, and in lesser amounts in most tissues and cell lines. -
Database links
- Entrez Gene: 4898 Human
- Entrez Gene: 230598 Mouse
- Omim: 602651 Human
- SwissProt: O43847 Human
- SwissProt: Q8BHG1 Mouse
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Alternative names
- EC 3.4.24.61 antibody
- hNRD1 antibody
- hNRD2 antibody
see all
Images
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Anti-Nardilysin antibody (ab154237) at 1/2000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 132 kDa
5% SDS PAGE -
Anti-Nardilysin antibody (ab154237) at 1/1000 dilution + Mouse kidney whole cell lysate at 50 µg
Predicted band size: 132 kDa
5% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling Nardilysin with ab154237 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154237 has not yet been referenced specifically in any publications.