Overview

  • Product name

    Anti-Nardilysin antibody - C-terminal
    See all Nardilysin primary antibodies
  • Description

    Rabbit polyclonal to Nardilysin - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment within Human Nardilysin (C terminal). The exact sequence is proprietary.
    Database link: O43847

  • Positive control

    • WB: NT2/D1 whole cell lysate; mouse kidney whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 132 kDa.

Target

  • Relevance

    A member of the metalloproteinase family M16, nardilysin is most similar to insulin degrading enzyme, and the bacterial peptidase pitrilysin. It cleaves peptide substrates on the N terminus of arginine residues in dibasic pairs. Nardilysin was first described as a processing enzyme of somatostatin 1-28, which is cleaved at the Arg-Lys paired amino acid location. Nardilysin was named N arginine dibasic convertase (NRD convertase) to reflect this cleavage, although it was later shown that the activity was due to aminopeptidase B acting as a heterodimer with nardilysin. In addition to somatostatin 1-28, nardilysin cleaves dynorphin A and a-neoendorphin. Nardilysin was first detected in the brain and testis, and later in the heart, skeletal muscle, and in lesser amounts in most tissues and cell lines.
  • Database links

  • Alternative names

    • EC 3.4.24.61 antibody
    • hNRD1 antibody
    • hNRD2 antibody
    • N arginine dibasic convertase antibody
    • Nardilysin precursor antibody
    • NRD C antibody
    • NRD convertase antibody
    • NRD1 antibody
    see all

Images

  • Anti-Nardilysin antibody - C-terminal (ab228745) at 1/1000 dilution + NT2/D1 (human malignant pluripotent embryonal carcinoma cell line from testis metastasis) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 132 kDa



    5% SDS-PAGE

  • Anti-Nardilysin antibody - C-terminal (ab228745) at 1/500 dilution + Mouse kidney whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 132 kDa



    5% SDS-PAGE

References

ab228745 has not yet been referenced specifically in any publications.

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