Anti-NARFL antibody (ab241215)

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Overview

  • Product name

    Anti-NARFL antibody
    See all NARFL primary antibodies

  • Description

    Rabbit polyclonal to NARFL

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Orangutan

  • Immunogen

    Synthetic peptide within Human NARFL aa 50-100. The exact sequence is proprietary.
    Database link: Q9H6Q4

  • Positive control

    • IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

8 - 15 µl/mg lysate

Target

  • Relevance

    NARFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.

  • Cellular localization

    Nuclear Cytosolic iron-sulfur protein assembly complex

  • Database links

    • Alternative names

      • Cytosolic Fe-S cluster assembly factor NARFL antibody
      • FLJ21988 antibody
      • HPRN antibody
      • IOP1 antibody
      • Iron only hydrogenase like protein 1 antibody
      • iron-only hydrogenase-like protein 1 antibody
      • LET1 like/JFP15 antibody
      • LET1L antibody
      • Nuclear prelamin A recognition factor like antibody
      • Nuclear prelamin A recognition factor like protein antibody
      • nuclear prelamin A recognition factor-like protein antibody
      • PRN antibody
      • Protein related to Narf antibody
      • uclear prelamin A recognition factor-like antibody
      see all

    Images

    • Immunoprecipitation - Anti-NARFL antibody (ab241215)
      Immunoprecipitation - Anti-NARFL antibody (ab241215)

      NARFL was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. ab241215 used for IP at 8 µl per reaction. For WB at a 1:400 dilution.

      Lane 1: ab241215 IP in HeLa whole cell lysate.
      Lane 2: Control IgG.

      Chemiluminescence detection: 30 seconds.

    References

    ab241215 has not yet been referenced specifically in any publications.

    Publishing research using ab241215? Please let us know so that we can cite the reference in this datasheet.

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