Key features and details
- Rabbit polyclonal to NARFL
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NARFL antibody
See all NARFL primary antibodies
DescriptionRabbit polyclonal to NARFL
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Orangutan
Synthetic peptide within Human NARFL aa 50-100. The exact sequence is proprietary.
Database link: Q9H6Q4
- IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
8 - 15 µl/mg lysate
RelevanceNARFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Cellular localizationNuclear Cytosolic iron-sulfur protein assembly complex
- Cytosolic Fe-S cluster assembly factor NARFL antibody
- FLJ21988 antibody
- HPRN antibody
NARFL was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer. ab241215 used for IP at 8 µl per reaction. For WB at a 1:400 dilution.
Lane 1: ab241215 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241215 has not yet been referenced specifically in any publications.