Anti-NARG1 antibody [434C2a] (ab60065)
Key features and details
- Mouse monoclonal [434C2a] to NARG1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-NARG1 antibody [434C2a]
See all NARG1 primary antibodies -
Description
Mouse monoclonal [434C2a] to NARG1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment (Human)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified using protein G column chromatography, from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
434C2a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
The NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter. -
Tissue specificity
Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. -
Sequence similarities
Contains 8 TPR repeats. -
Post-translational
modificationsCleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes. - Information by UniProt
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Alternative names
- 5730450D16Rik antibody
- 6330400I15 antibody
- ASTBDN antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab60065 has been referenced in 2 publications.
- Kweon HY et al. Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway. Elife 10:N/A (2021). PubMed: 34355692
- Dörfel MJ et al. Proteomic and genomic characterization of a yeast model for Ogden syndrome. Yeast 34:19-37 (2017). WB . PubMed: 27668839