Key features and details
- Mouse monoclonal [434C2a] to NARG1
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.0225% Potassium chloride, 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 0.812% Sodium chloride, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified using protein G column chromatography, from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Our Abpromise guarantee covers the use of ab60065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Detects a band of approximately 32 kDa for the recombinant protein fragment (predicted molecular weight: 101 kDa for the full length protein).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThe NAA10-NAA15 complex displays alpha (N-terminal) acetyltransferase activity that may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.
Tissue specificityExpressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.
Sequence similaritiesContains 8 TPR repeats.
modificationsCleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.
Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.
- Information by UniProt
- 5730450D16Rik antibody
- 6330400I15 antibody
- ASTBDN antibody
ab60065 has been referenced in 1 publication.
- Dörfel MJ et al. Proteomic and genomic characterization of a yeast model for Ogden syndrome. Yeast 34:19-37 (2017). WB . PubMed: 27668839