Overview

  • Product name

    Anti-NARS antibody [EPR8221]
    See all NARS primary antibodies
  • Description

    Rabbit monoclonal [EPR8221] to NARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NARS aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver tissue lysate, HepG2, K562, and HT-1080 cell lysates; Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 7.09 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8221
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab129162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 63 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Sequence similarities

      Belongs to the class-II aminoacyl-tRNA synthetase family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3010001M15Rik antibody
      • AA960128 antibody
      • AsnRS antibody
      • ASNS antibody
      • Asparagine tRNA ligase 1, cytoplasmic antibody
      • Asparagine tRNA ligase antibody
      • Asparagine--tRNA ligase antibody
      • Asparaginyl tRNA synthetase antibody
      • Asparaginyl-tRNA synthetase antibody
      • C78150 antibody
      • cytoplasmic antibody
      • EC 6.1.1.22 antibody
      • LRRGT00113 antibody
      • MGC116236 antibody
      • Nars antibody
      • NARS1 antibody
      • NRS antibody
      • SYNC_HUMAN antibody
      see all

    Images

    • ab129162, at 1/50 dilution, staining NARS in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-NARS antibody [EPR8221] (ab129162) at 1/10000 dilution

      Lane 1 : Human fetal liver tissue lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 63 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Hsu CH  et al. Identification and Characterization of Potential Biomarkers by Quantitative Tissue Proteomics of Primary Lung Adenocarcinoma. Mol Cell Proteomics 15:2396-410 (2016). Read more (PubMed: 27161446) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129162.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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