Product nameAnti-NASP antibody [EPR12827]
See all NASP primary antibodies
DescriptionRabbit monoclonal [EPR12827] to NASP
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NASP aa 50-150. The exact sequence is proprietary.
Database link: P49321
- HeLa, Caco2, 293 or NTERA2/D1 lysate, 293 or HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 85 kDa.|
|ICC/IF||1/100 - 1/250.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/40 - 1/60.|
FunctionRequired for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.
Tissue specificityIsoform 1 is testis- and sperm-specific.
Sequence similaritiesBelongs to the NASP family.
Contains 3 TPR repeats.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- 5033430J04Rik antibody
- AI131596 antibody
- AI317140 antibody
All lanes : Anti-NASP antibody [EPR12827] (ab181169) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : Caco2 lysate
Lane 3 : 293 lysate
Lane 4 : NTERA2/D1 lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 85 kDa
Detection of Human NASP by Western Blot of Immunoprecipitate. Caco2 whole cell lysate loaded, ab181169 immunoprecipitated NASP at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary used at a 1/1000 dilution.
Flow cytometry analysis of HeLa cells using ab181169 at a 1/40 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/40 dilution.
Immunofluorescence analysis of 293 cells (fixative 4% paraformaldehyde) labeling NASP with ab181169 at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.
ab181169 has not yet been referenced specifically in any publications.