Name: Anti-NAT10 antibody [EPR18663]
Brand: RabMAb
SKU: ab194297
Rating: 5 (1 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18663] to NAT10
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-NAT10 antibody [EPR18663]
See all NAT10 primary antibodies
Description

Rabbit monoclonal [EPR18663] to NAT10
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain and fetal heart lysates; Mouse brain, rat heart and rat spleen lysates. IHC-P: Human colon, mouse stomach and rat colon tissues. ICC/IF: HeLa and NIH/3T3 cells. IP: HeLa cell lysate. Flow Cyt (intra): NIH/3T3 cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18663
Isotype

IgG
Research areas

Alternative Versions
- Anti-NAT10 antibody [EPR18663] - BSA and Azide free (ab251186)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human NAT10 protein (ab163025)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/600.
WB		1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ICC/IF	(1)	1/2000.
IP		1/80.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
Flow Cyt (Intra) 1/600.
WB 1/2000. Detects a band of approximately 116 kDa (predicted molecular weight: 116 kDa).
ICC/IF 1/2000.
IP 1/80.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, and may also regulate telomerase function by affecting the balance of telomerase subunit assembly, disassembly, and localization. Acetylates alpha-tubulin, which may affect microtubule stability and cell division.
Sequence similarities

Belongs to the UPF0202 family.
Contains 1 N-acetyltransferase domain.
Cellular localization

Nucleus > nucleolus. Nucleolar in interphase and redistributes to the perichromosomal layer and to the midbody during telophase.
Target information above from: UniProt accession Q9H0A0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 55226 Human
- Entrez Gene: 98956 Mouse
- Entrez Gene: 311257 Rat
- Omim: 609221 Human
- SwissProt: Q9H0A0 Human
- SwissProt: Q8K224 Mouse
- Unigene: 577281 Human
- Unigene: 288005 Mouse
Alternative names
- ac4C enzyme antibody
- ac4C writer antibody
- DKFZp434C116 antibody
- FLJ10774 antibody
- FLJ12179 antibody
- FLJ23850 antibody
- hALP antibody
- KIAA1709 antibody
- N acetyltransferase 10 antibody
- N acetyltransferase 10 GCN5 related antibody
- N acetyltransferase like antibody
- N acetyltransferase like protein antibody
- N-acetyltransferase 10 antibody
- NAT10 antibody
- NAT10_HUMAN antibody
- NET43 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on HeLa cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:-
-ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)

All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 30 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on rat colon tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-NAT10 antibody [EPR18663] (ab194297)

Intracellular Flow Cytometry analysis of NIH/3T3 (mouse embryo) cells labelling NAT10 (red) with purified ab194297 at dilution of 1/600. The secondary antibody used was Alexa Fluorr^® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody used was Rabbit Monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
Western blot - Anti-NAT10 antibody [EPR18663] (ab194297)

All lanes : Anti-NAT10 antibody [EPR18663] (ab194297) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 116 kDa
Observed band size: 116 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on Human colon tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NAT10 antibody [EPR18663] (ab194297)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling NAT10 with ab194297 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on NIH/3T3 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows:-
-ve control 1: ab194297 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAT10 antibody [EPR18663] (ab194297)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling NAT10 with ab194297 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleus staining on mouse stomach tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-NAT10 antibody [EPR18663] (ab194297)

NAT10 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate with ab194297 at 1/80 dilution.

Lane 1: HeLa cell lysate 10ug (Input).

Lane 2: ab194297 IP in HeLa cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194297 in HeLa cell lysate.

Western blot was performed from the immunoprecipitate using ab194297 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-NAT10 antibody [EPR18663] (ab194297)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet download

Download

Publishing research using ab194297? Please let us know so that we can cite the reference in this datasheet.

ab194297 has been referenced in 8 publications.

Fei X et al. Amniotic fluid mesenchymal stem cells repair mouse corneal cold injury by promoting mRNA N4-acetylcytidine modification and ETV4/JUN/CCND2 signal axis activation. Hum Cell 34:86-98 (2021). PubMed: 33010000
Zhu Z et al. NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1. Stem Cells Int 2021:8833527 (2021). PubMed: 33953754
Zhou L et al. Doxorubicin restrains osteogenesis and promotes osteoclastogenesis in vitro. Am J Transl Res 12:5640-5654 (2020). PubMed: 33042445
Liu HY et al. Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Nucleic Acids Res 48:3638-3656 (2020). PubMed: 32112098
Li G et al. Long non-coding RNA-H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA-149/SDF-1 axis. J Cell Mol Med 24:4944-4955 (2020). PubMed: 32198976
Choi YJ et al. Tubastatin A inhibits HDAC and Sirtuin activity rather than being a HDAC6-specific inhibitor in mouse oocytes. Aging (Albany NY) 11:1759-1777 (2019). PubMed: 30913540
Jia S et al. Calcitonin gene-related peptide enhances osteogenic differentiation and recruitment of bone marrow mesenchymal stem cells in rats. Exp Ther Med 18:1039-1046 (2019). PubMed: 31316600
Wang W et al. Eldecalcitol, an active vitamin D analog, effectively prevents cyclophosphamide-induced osteoporosis in rats. Exp Ther Med 18:1571-1580 (2019). PubMed: 31410111

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (PC12, a cell line derived from a pheochromocytoma)

Permeabilization

Yes - 0.1% TritonX-100

Specification

PC12, a cell line derived from a pheochromocytoma

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Sep 18 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Datasheet download

Certificate of Compliance

References (8)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-NAT10 antibody [EPR18663]