Key features and details
- Rabbit polyclonal to NAT13
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NAT13 antibody
See all NAT13 primary antibodies
DescriptionRabbit polyclonal to NAT13
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow, Orangutan
Synthetic peptide within Human NAT13 aa 119-169. The exact sequence is proprietary.
Database link: Q9GZZ1
- WB: HeLa, HEK-293T, jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: NAT13 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to NAT13 immobilized on solid support
Our Abpromise guarantee covers the use of ab241487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 μl/mg lysate
|WB||1/1000. Predicted molecular weight: 19 kDa.|
FunctionProbable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity.
Sequence similaritiesBelongs to the acetyltransferase family. GNAT subfamily.
Contains 1 N-acetyltransferase domain.
- Information by UniProt
- MAK3 antibody
- FLJ13194 antibody
- hNAT5 antibody
NAT13 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241487 at 20 µL per reaction. Western blot was perfromed on the immunoprecipitate using ab241487 at 1/250 dilution.
Lane 1: ab241487 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-NAT13 antibody (ab241487) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 10 seconds
Lysates prepared in NETN buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241487 has not yet been referenced specifically in any publications.