Overview

  • Product name

  • Description

    Rabbit polyclonal to NAT13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide within Human NAT13 aa 119-169. The exact sequence is proprietary.
    Database link: Q9GZZ1

  • Positive control

    • WB: HeLa, HEK-293T, jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: NAT13 IP in HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibody was affinity purified using an epitope specific to NAT13 immobilized on solid support
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

20 μl/mg lysate

 

WB 1/1000. Predicted molecular weight: 19 kDa.

Target

  • Function

    Probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity.
  • Sequence similarities

    Belongs to the acetyltransferase family. GNAT subfamily.
    Contains 1 N-acetyltransferase domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • MAK3 antibody
    • FLJ13194 antibody
    • hNAT5 antibody
    • hSAN antibody
    • Mak3 homolog (S. cerevisiae) antibody
    • Mak3 homolog antibody
    • N-acetyltransferase 13 (GCN5-related) antibody
    • N-acetyltransferase 13 antibody
    • N-acetyltransferase 5 antibody
    • N-acetyltransferase san homolog antibody
    • N-alpha-acetyltransferase 50 antibody
    • N-alpha-acetyltransferase 50, NatE catalytic subunit antibody
    • Naa50 antibody
    • NAA50_HUMAN antibody
    • NAT 5 antibody
    • NAT13 antibody
    • NAT5 antibody
    • NatE catalytic subunit antibody
    • San antibody
    see all

Images

  • NAT13 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241487 at 20 µL per reaction. Western blot was perfromed on the immunoprecipitate using ab241487 at 1/250 dilution.

    Lane 1: ab241487 IP in HEK-293T whole cell lysate.
    Lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

     

     

  • All lanes : Anti-NAT13 antibody (ab241487) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 19 kDa


    Exposure time: 10 seconds


    Lysates prepared in NETN buffer.

References

ab241487 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab241487.
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