Recombinant
RabMAb

Recombinant Anti-NAT2/AT-2 antibody [EPR15856] (ab194114)

Overview

  • Product name

    Anti-NAT2/AT-2 antibody [EPR15856]
    See all NAT2/AT-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15856] to NAT2/AT-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    corresponding to Human NAT2/AT-2 aa 250 to the C-terminus.
    Database link: P11245

  • Positive control

    • Human fetal liver and colon cancer lysates; HepG2 cells.
  • General notes

     

     

     This product was previously labelled as NAT2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab194114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/50.

Target

  • Function

    Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens.
  • Sequence similarities

    Belongs to the arylamine N-acetyltransferase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AAC2 antibody
    • ARY2_HUMAN antibody
    • Arylamide acetylase 2 (N-acetyltransferase 2, isoniazid inactivation) antibody
    • Arylamide acetylase 2 antibody
    • Arylamine N-acetyltransferase 2 antibody
    • HGNC:7646 antibody
    • N-acetyltransferase 2 (arylamine N-acetyltransferase) antibody
    • N-acetyltransferase type 2 antibody
    • NAT-2 antibody
    • NAT2 antibody
    • PNAT antibody
    • Polymorphic arylamine N-acetyltransferase antibody
    see all

Images

  • Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/5000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-NAT2/AT-2 antibody [EPR15856] (ab194114) at 1/1000 dilution + Human colon cancer lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 cells labeling NAT2/AT-2 with ab194114 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

References

ab194114 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab194114.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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