Native B. pertussis Bordetella pertussis toxin/ptxD protein (ab124299)

Overview

  • Product name

    Native B. pertussis Bordetella pertussis toxin/ptxD protein
  • Protein length

    Full length protein

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
    • Accession
    • Species

      Bordetella pertussis
    • Molecular weight

      30 kDa
    • Amino acids

      1 to 269

Specifications

Our Abpromise guarantee covers the use of ab124299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.

     This product was previously labelled as Bordetella pertussis toxin

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

    pH: 7.20
    Constituents: 0.8% Sodium phosphate, 2.9% Sodium chloride

  • Reconstitution
    Reconstitution of one vial with 400 µl distilled water will give a solution containing 100µg of pertussis toxin, 0.05M sodium phosphate and 0.5M sodium chloride at pH 7.2.

General Info

  • Alternative names

    • IAP S1
    • Islet-activating protein S1
    • NAD-dependent ADP-ribosyltransferase
  • Relevance

    Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5

References

ab124299 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

We carry the Bordetella pertussis toxin protein (ab124299: https://www.abcam.com/bordetella-pertussis-toxin-protein-ab124299.html); however, it is unconjugated.

I have attached our protocol book which includes recommendations for antigen coating for indirect ELISA. To summarize, the antigen should be diluted to a final concentration of 20 ug/ml in PBS, and the microtiter plate should be coated with 50ul of the antigen dilution in the top wells of the plate. The plate should then be incubated for 2 hours at room temperature or 4ºC overnight.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up