Native B. pertussis Bordetella pertussis toxin/ptxD protein (ab124299)

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Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Native B. pertussis Bordetella pertussis toxin/ptxD protein
    See all Bordetella pertussis toxin/ptxD proteins and peptides

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native

  • Accession

    P04977

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Native

    • Species

      Bordetella pertussis

    • Predicted molecular weight

      30 kDa

    • Amino acids

      1 to 269

Specifications

Our Abpromise guarantee covers the use of ab124299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilized

  • Additional notes

    This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.

     This product was previously labelled as Bordetella pertussis toxin

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

    pH: 7
    Constituents: 0.8% Sodium phosphate, 2.9% Sodium chloride

  • Reconstitution
    Reconstitution of one vial with 400 µl distilled water will give a solution containing 100µg of pertussis toxin, 0.05M sodium phosphate and 0.5M sodium chloride at pH 7.2.

General Info

  • Alternative names

    • IAP S1
    • Islet-activating protein S1
    • NAD-dependent ADP-ribosyltransferase

  • Relevance

    Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab124299? Please let us know so that we can cite the reference in this datasheet.

ab124299 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Dear abcam:



We have a customer that like develop a kit of indirect ELISA. He required antigen of Adacel Boost vaccine, in specific

1) fimbrial agglutinogen 2 or fimbriae 2 antigen of Bordetella pertusis

2) pertactin antigen (PRN) is a protein of 69 http://es.wikipedia.org/wiki/Unidad_de_masa_at%C3%B3mica of http://es.wikipedia.org/wiki/Bordetella_pertussis



Do you have that be conjugated with BSA or other carrier the antigens for ELISA? Do you know the quantity aproxi. that the client could use of antigen for each well?

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Answer

Thank you for contacting us.

We carry the Bordetella pertussis toxin protein (ab124299: https://www.abcam.com/bordetella-pertussis-toxin-protein-ab124299.html); however, it is unconjugated.

I have attached our protocol book which includes recommendations for antigen coating for indirect ELISA. To summarize, the antigen should be diluted to a final concentration of 20 ug/ml in PBS, and the microtiter plate should be coated with 50ul of the antigen dilution in the top wells of the plate. The plate should then be incubated for 2 hours at room temperature or 4ºC overnight.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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