Overview

Description

  • Nature
    Native
  • Source
    Native

Specifications

Our Abpromise guarantee covers the use of ab9936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    SDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
  • Form
    Liquid
  • Additional notes
    Native Human Cardiac Troponin-ISDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 48% Urea, 0.117% Beta mercaptoethanol, 0.79% Tris HCl, 0.0292% EDTA

General Info

  • Alternative names
    • cardiac muscle
    • Cardiac troponin I
    • cardiomyopathy, dilated 2A (autosomal recessive)
    • Cardiomyopathy, familial hypertrophic, 7, included
    • CMD1FF
    • CMD2A
    • CMH7
    • cTnI
    • Familial hypertrophic cardiomyopathy 7
    • MGC116817
    • RCM1
    • Tn1
    • Tni
    • TNN I3
    • TNNC 1
    • TNNC1
    • TNNI3
    • TNNI3_HUMAN
    • Troponin I
    • Troponin I cardiac
    • Troponin I cardiac muscle
    • Troponin I cardiac muscle isoform
    • Troponin I type 3 cardiac
    • troponin I, cardiac 3
    • TroponinI
    • Ttroponin I type 3 (cardiac)
    see all
  • Function
    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease
    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the troponin I family.
  • Information by UniProt

Images

  • Native Cardiac Troponin I protein (ab9936) at 2.7 µg (Coomassie blue staining of SDS gel)
    Performed under reducing conditions.

    Observed band size: 24 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa (possible dimer)

References

This product has been referenced in:
  • Krupin O & Berini P Long-Range Surface Plasmon-Polariton Waveguide Biosensors for Human Cardiac Troponin I Detection. Sensors (Basel) 19:N/A (2019). Read more (PubMed: 30717341) »
  • Sinha A  et al. An integrated microfluidic platform to perform uninterrupted SELEX cycles to screen affinity reagents specific to cardiovascular biomarkers. Biosens Bioelectron 122:104-112 (2018). Read more (PubMed: 30245322) »
See all 2 Publications for this product

Customer reviews and Q&As

11-14 of 14 Abreviews or Q&A

Answer

Thank you for your inquiry. I heard back from the supplying lab that this protein is purified using ion-exchange and two rounds of calcium-dependent troponin-C affinity chromatography in 8M urea solution. Therefore, little else binds to the TnC column. We use transplant quality human tissue as source material. Any, low molecular weight bands seen on SDS PAGE are minor degradation fragments of TnI that result from proteolysis that occurs in the heart prior to freezing and this is unavoidable. As far as endotoxin is concerned, because the protein is sourced from human tissue using multiple chromatography columns we make no attempt to control levels. If we did, the end product would be prohibitively expensive. Unfortunately, the lab did not have recommendations to improve the purity as they consider it to be highly pure and they do not offer it in any other formats. I am sorry that this information could not be more helpful, but please let me know if you have any other questions.  

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Answer

Thank you for your email. The ab9936 product is a peptide which can be used to block antibodies ab7829, ab8290 and ab8291. This peptide can be used to block these antibodies irrespective of applications e.g. It is suitable to use with antibodies in Immunoprecipitation, western blot, Immunohistochemistry, ELSIA & also other blocking assays. I hope this information will be helpful. Should you have any other question please do not hesitate to ask.

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Answer

Thank you for your email. We have updated the datasheet of ab10236; few of the match pairs are no longer available in our catalogue so we have removed these from the datasheet. We recommend using ab10236 as a capture ab along with ab10239 as a detection antibody. In general many general protocols could be used with our cTnI antibodies and the optimal working concentrations should be determined separately for each application by the user. In our own lab we are using a special two-step immunofluorometric assay and streptavidin-coated plates with biotinylated capture MAbs and detection MAbs which are conjugated with stable europium chelate. The MAb concentrations in this kind of assay are different than what are needed in some other kind of assay. With very general protocol using PBS buffers and HRP detection method I can only recommend to start testing with something like 5 ug/ml MAb concentration for capture MAb and 0.5-1 ug/ml concentration for HRP-conjugated detection MAb and then to start optimization from there. Regarding pH I would recommend 7.4. I hope that this information is helpful to you. Should you have any question please do not hesitate to contact me.

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Answer

Troponin-I is quite stable in the urea buffer in which it is provided if maintained at 2-8oC for several days but we recommend that it be stored frozen at -20oC. Vortexing won't harm the protein provided it isn't excessive. TnI is a fragile and hydrophobic protein in most non-urea buffers. If you have any additional questions, please contact us again.

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11-14 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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