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How is the purity of this protein determined?
Asked on Mar 09 2012
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The purity is determined by observation of a stained SDS-PAGE gel. The information I received from our source of the cardiac troponin protein is as follows:
Purity is estimated visually from the SDS PAGE gel. It probably exceeds 95%. Troponin-I is very susceptible to proteolysis and lower mwt bands on SDS PAGE represent TnI fragments (confirmed by western blot). The proteolysis occurs during the time the heart is in cold storage. With human hearts, it is extremely difficult to obtain human hearts that have been frozen immediately after death.
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Answered on Mar 09 2012