Question (39844) | Native Cardiac Troponin I protein (ab9936)

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Question

Dear,   It is suitable for ELISA to store the protein ab9936 with the storage buffer that you recommend (8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0))? Thank you,

Answer

Thank you for contacting Abcam.

I would not recommend using a product in ELISA with a storage buffer which contains8 M Urea, 15 mM ß-mercaptoethano as I would be wary of the buffer denaturing your antibody. I would recommend using a peptide which has a non-reducing buffer such as ab50822;https://www.abcam.com/cardiac-Troponin-I-protein-ab50822.html.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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