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Dear, It is suitable for ELISA to store the protein ab9936 with the storage buffer that you recommend (8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0))? Thank you,
Asked on Mar 13 2012
Thank you for contacting Abcam.
I would not recommend using a product in ELISA with a storage buffer which contains8 M Urea, 15 mM ß-mercaptoethano as I would be wary of the buffer denaturing your antibody. I would recommend using a peptide which has a non-reducing buffer such as ab50822;https://www.abcam.com/cardiac-Troponin-I-protein-ab50822.html.
I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.
Answered on Mar 13 2012