Question (40004) | Native Cardiac Troponin I protein (ab9936)

Go to datasheet (ab9936)

Question

Currently, I am using ab9936 for ELISA because you recommend it to me in the past. So, maybe it is better to store ab9936 in :   Preservative: 0.025% Sodium Azide Constituents: 50% Glycerol, 12.5µg/ml Aprotinin, 250mM Sodium chloride, 20mM Tris HCl, 2mM Magnesium chloride, 2mM Calcium chloride   Isn’t it?

Answer

Thank you for your reply.

Further investigation into this protein revealed that this is very tightly bound and hydrophobic (http://www.rcsb.org/pdb/explore/explore.do?structureId=1MXL). As such the full peptide will need to be stored and used in a reduced form to be effective. Ab50822 is a single strand peptide and do not require these conditions.

While using this in ELISA is not ideal, the protein structure itself makes this a necessity. A shorter incubation at room temperature, one hour, with the sample may provide better results.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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