Native Chicken Deglycosylated Avidin protein (Allophycocyanin) (ab136216)

Overview

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
    • Accession
    • Species

      Chicken
    • Molecular weight

      60 kDa
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm

Associated products

Specifications

Our Abpromise guarantee covers the use of ab136216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    ab136216 binds D-Biotin.
  • Applications

    ELISA

    Western blot

    Flow Cytometry

  • Purity

    > 95 % SDS-PAGE.

  • Form

    Liquid
  • Additional notes

    Deglycosylated acidic avidin has higher affinity for biotin than standard avidin and therefore reduces background staining. ab136216 binds D-Biotin.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark.

    Preservative: 0.1% Sodium azide
    Constituent: PBS

    Contains a stabilizing agent(Sucrose, the percentage is considered proprietary)

General Info

  • Alternative names

    • AVD
    • AVID_CHICK
    • Avidin
  • Function

    The biological function of avidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of avidin).
  • Tissue specificity

    Synthesized in hen oviduct and concentrated in egg white (where it represent 0.05% of the total protein).
  • Sequence similarities

    Belongs to the avidin/streptavidin family.
    Contains 1 avidin-like domain.
  • Post-translational
    modifications

    N-linked glycan at Asn-41 consists of GlcNAc(beta1-2)Man(alpha1-3)[GlcNAc(beta1-4)][Man(alpha1-?)Man(alpha1-6)] Man(beta1-4)GlcNAc(beta1-4)GlcNAc.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

ab136216 has not yet been referenced specifically in any publications.

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