Native Clostridium difficile Toxin B protein (ab124001)
Key features and details
- Expression system: Native
- Purity: > 95% Affinity purified
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Native Clostridium difficile Toxin B protein -
Biological activity
Measured by vercytotoxicity assay with 2 pg/ml as lowest point at which rounded cells are observed.
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Purity
> 95 % Affinity purified.
Clostridium difficile Toxin B has been highly purified. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Predicted molecular weight
270 kDa -
Amino acids
2 to 2366
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab124001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Lyophilized -
Additional notes
This protein is derived from a pathogenic organism, and may be involved in a disease process, consequently exposure may have adverse health effects.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Functional Studies Protocol
1.1 Vero cells are plated out the night before on a 96-well plate at a concentration of 20K cells per well (in 100ml), then incubated at 37°C, 5% CO2. The following day they should be lightly confluent.
1.2 Lyophilised toxin/toxoid is resuspended as per the instructions in the certificate of analysis, and diluted in duplicate to 0.1mg/ml in DMEM (200ml total) without FBS in the first well of a non-TC-treated 96-well plate. A serial 5-fold dilution is performed (40ul in 160ul) in the same media using a multichannel for 11 dilutions in total, leaving the 12th dilution as a media control.
1.3 20ul of each dilution is then pipetted onto each well of the pre-prepared Vero cells and then incubated at 37°C, 5% CO2 for 24 hours.
1.4 The following day, the cells are examined using an inverted phase contrast tissue culture microscope. Cells with active toxin on should be rounded, cells without significant toxicity should look like the control cells. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Please see notes section.
pH: 7
Constituents: 1.19% HEPES, 5% Sucrose, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute in 250µl sterile distilled water. Swirl or pipette gently to mix. Vortexing promotes aggregation. Store the lyophilised vial between +2°C to +8°C for up to 1 year from date of receipt. Store the reconstituted liquid between +2°C to +8°C for up to a month. Reconstituted liquid can be frozen immediately and stored at -80°C to extend stability to 1 year.
General Info
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Alternative names
- C. difficile Toxin B
- Clostridium difficile Toxin B
- Cytotoxin B
see all -
Relevance
Clostridium difficile Toxin B (tcdB or ToxB) is a glucosyltranferase which is known to innactivate Rho, Cdc42 and Rac within target cells. This toxin is encoded on a pathogenicity region of the C. difficile chromossome and is expressed during the log and stationary phases of growth in response to a variety of environmental stimuli. -
Cellular localization
Secreted
Images
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SDS-PAGE - Native Clostridium difficile Toxin B protein (ab124001)SDS-PAGE showing ab124001 at 270 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab124001 has been referenced in 4 publications.
- Magnotti F et al. Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. EMBO Mol Med 11:e10547 (2019). PubMed: 31589380
- Hartley-Tassell LE et al. Lectin activity of the TcdA and TcdB toxins of Clostridium difficile. Infect Immun N/A:N/A (2018). PubMed: 30530621
- Moghaddas F et al. A novel Pyrin-Associated Autoinflammation with Neutrophilic Dermatosis mutation further defines 14-3-3 binding of pyrin and distinction to Familial Mediterranean Fever. Ann Rheum Dis 76:2085-2094 (2017). PubMed: 28835462
- Hutton ML et al. Bovine antibodies targeting primary and recurrent Clostridium difficile disease are a potent antibiotic alternative. Sci Rep 7:3665 (2017). Neutralising ; Clostridium difficile . PubMed: 28623367