Native Collagen V protein (ab7530)

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Overview

Description

  • Nature
    Native
  • Source
    Native

Associated products

Specifications

Our Abpromise guarantee covers the use of ab7530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    Collagen taken from bovine species.
  • Applications

    Western blot

    Immunoprecipitation

    ELISA

  • Form
    Liquid
  • Additional notes

    This product reacts with anti-Collagen Type V. Reaction with anti-Collagen I, II, III, IV or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

    Collagen taken from bovine species.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 4.50
    Constituent: 0.82% Sodium acetate

General Info

  • Alternative names
    • AB collagen
    • Alpha 1 type V collagen
    • Alpha 2 type V collagen
    • CO5A1_HUMAN
    • COL5A1
    • Col5A2
    • COL5A2 protein
    • Col5A3
    • Collagen alpha 1(V) chain
    • Collagen alpha 2 (V) chain precursor
    • Collagen alpha 2(V) chain
    • Collagen alpha 3(V) chain
    • Collagen alpha-1(V) chain
    • Collagen fetal membrane A polypeptide
    • Collagen type V alpha 1
    • Collagen type V alpha 2
    • Collagen type V alpha 3
    • Collagen V alpha 2 polypeptide
    • CollagenV
    • MGC105115
    • OTTHUMP00000064637
    • Pro alpha 1 type V collagen
    • Pro alpha 3(V) collagen
    • Procollagen alpha 2(V)
    • Type V preprocollagen alpha 2 chain
    see all
  • Function
    Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
  • Involvement in disease
    Ehlers-Danlos syndrome 1
    Ehlers-Danlos syndrome 2
  • Sequence similarities
    Belongs to the fibrillar collagen family.
    Contains 1 fibrillar collagen NC1 domain.
    Contains 1 laminin G-like domain.
  • Domain
    The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function.
  • Post-translational
    modifications
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Sulfated on 40% of tyrosines.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

References

ab7530 has not yet been referenced specifically in any publications.

Publishing research using ab7530? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

We are currently using human collagen V from another vendor for ELISAs but have recently had problems with small molecular weight contaminants which seem to be affecting our ELISA results. I am curious to know how clean your collagen V preps are ie is SDS-PAGE routinely done to screen for contaminants?

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Answer

Thank you for your enquiry. This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. I enquired with our source for this protein and the originator said that it is sterile filtered and kept frozen. No routine check-up within its shelf life is done unless any problem is reported. Please be assured that our policy at Abcam is that if a product does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please contact us again if you have any additional questions.

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Question

Collagen V has various isoforms, denoted A1, A2, etc. Can you tell me the cross reactivity of this antibody among the isoforms? I am looking for specific antibodies to Col V A2 Thank you very much.

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Answer

The immunogen to make this antibody is derived from human and bovine placenta. The major isoform comprises two proalpha1 chains and one proalpha2 chain. The second isoform comprises proalpha1, proalpha2 and proalpha3 chains.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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