Native Cow 20S Proteasome protein (ab218005)

Description

  • Product name

    Native Cow 20S Proteasome protein
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Cow
    • Predicted molecular weight

      700 kDa
    • Additional sequence information

      Purified from bovine red blood cells.

Specifications

Our Abpromise guarantee covers the use of ab218005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.24% Tris, 10% Glycerol, 0.04% Beta mercaptoethanol, 0.03% EDTA, 0.12% Sodium chloride

General Info

  • Alternative names

    • 20S core particle
  • Relevance

    The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.

Images

  • Coomassie-stained native-PAGE analysis of ab218005 (5 μg).

  • Coomassie-stained native-PAGE analysis of ab218005 (5 μg).

  • Activation of 5 nM ab218005 by 25 nM PA28β, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

  • Activation of 5 nM ab218005 by 25 nM PA28α, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

  • Activation of 5 nM ab218005 by 25 nM PA700, the proteasome activity was assayed by using 50 µM Suc-LLVY-AMC as the substrate. The AMC fluorescence was monitored by a plate reader with excitation and emission filters of 360±40 nm and 460±30 nm, respectively.

References

ab218005 has not yet been referenced specifically in any publications.

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