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  1. Link

    native-cow-collagen-i-protein-ab7526.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Proteins Collagen
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Native Cow Collagen I protein (ab7526)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)

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ELISA - Native Cow Collagen I protein (ab7526)

    Key features and details

    • Expression system: Native
    • Suitable for: ELISA, IP, WB

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    Description

    • Product name

      Native Cow Collagen I protein
      See all Collagen I proteins and peptides
    • Expression system

      Native
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Native
      • Species

        Cow

    Specifications

    Our Abpromise guarantee covers the use of ab7526 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Immunoprecipitation

      Western blot

    • Form

      Liquid
    • Additional notes

      This product has been prepared from Bovine Placenta and is chromatographically and immunologically pure. It reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

      This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      Preservative: 0.01% Sodium azide
      Constituent: 3% Acetic acid

    General Info

    • Alternative names

      • Alpha 1 type I collagen
      • Alpha 2 type I collagen
      • alpha 2 type I procollagen
      • alpha 2(I) procollagen
      • alpha 2(I)-collagen
      • Alpha-1 type I collagen
      • alpha1(I) procollagen
      • CO1A1_HUMAN
      • COL1A1
      • COL1A2
      • collagen 1
      • collagen alpha 1 chain type I
      • Collagen alpha-1(I) chain
      • collagen alpha-1(I) chain preproprotein
      • Collagen I alpha 1 polypeptide
      • Collagen I alpha 2 polypeptide
      • collagen of skin, tendon and bone, alpha-1 chain
      • collagen of skin, tendon and bone, alpha-2 chain
      • Collagen type I alpha 1
      • Collagen type I alpha 2
      • EDSC
      • OI1
      • OI2
      • OI3
      • OI4
      • pro-alpha-1 collagen type 1
      • type I proalpha 1
      • Type I procollagen
      • type I procollagen alpha 1 chain
      see all
    • Function

      Type I collagen is a member of group I collagen (fibrillar forming collagen).
    • Tissue specificity

      Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
    • Involvement in disease

      Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
      Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
      Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 2A (OI2A) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
      Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
      Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
      Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.
    • Sequence similarities

      Belongs to the fibrillar collagen family.
      Contains 1 fibrillar collagen NC1 domain.
      Contains 1 VWFC domain.
    • Post-translational
      modifications

      Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some of the chains.
      O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P02452 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • ELISA - Native Cow Collagen I protein (ab7526)
      ELISA - Native Cow Collagen I protein (ab7526)Image courtesy of an anonymous Abreview.
      ab7526 used to generate a standard curve for ELISA, diluted to a concentration of 4, 2, 1 and 0.5 ug.
      Samples were from bovine LD muscle.

      ab34710 was used at a 1/1000 dilution as the primary antibody.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab7526? Please let us know so that we can cite the reference in this datasheet.

    ab7526 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    ELISA Abreview for Collagen I protein

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA

    Review text: I used this collagen protein to generate my standard curve for ELISA, diluted to a concentration of 4, 2, 1, 0.5 ug
    Sample: Cow Tissue sections (Beef LD muscle)
    Specification: Beef LD muscle
    Blocking step: BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Type: Sandwich (Capture)

    Other product details
    Incubation time: 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: carbonated coating buffer

    Primary antibody (in addition to 'Collagen I protein')
    Primary antibody: Abcam primary antibody: Anti-Collagen I antibody (ab34710)
    Dilution: 1/1000

    Secondary antibody
    Name: Abcam antibody:(not specified yet)
    Dilution: 1/1000

    Additional data
    Additional Notes: Primary antibody: Ab34710 rabbit polyclonal to collagen I (1:1000 in carbobate coating buffer) add 100 ul to wells and incubate at 37C for 2 h
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 12 2011

    Question

    hello


    May I konw the accurate purity of the Collagen I protein (ab7526)?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2012

    Answer

    The collagen protein ab7526 is purified from the full length native bovine protein by a process involving limited pepsin digestion. The exact purity for this product is not determined.

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2012

    Question

    Is this product:
    1. type I collagen extracted from equine/bovine/porcine tendon,

    2. telopeptides free

    3. supplied as acetic gel (an aqueous acetic buffer solution, pH= 3.5 containing

    1 wt% of pure collagen)

    Read More

    Abcam community

    Verified customer

    Asked on Feb 23 2012

    Answer

    The bovine type I collagenab7526 is prepared by a proprietary method of limited pepsin digestion and selective salt precipitation and is supplied in a sodium acetate buffer containing sodium azide. However it is not tested to be free of telepeptides.

    Read More

    Abcam Scientific Support

    Answered on Feb 23 2012

    Question

    Our customer has some questions to your Collagen I, AB7526. Can you tell more about the protein structure. Is it the intact protein? Are telopeptides still remain? Is the protein present in a triplehelix?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 10 2004

    Answer

    Thank you for your enquiry. It is a native protein extracted by pepsin digestion. The basic structure should still remain.

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2004

    Question

    Hi, I am wondering if this anti-human Collagen type I antibody will work to detect collagen type I from whole cell lysates in regular SDS-PAGE (i.e.: in denaturing and dissociating conditions)?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2004

    Answer

    Thank you for your enquiry and your interest in our product. We recommend non-denaturing and non-dissociating conditions. Other conditions are not supported.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2004

    Question

    Customer would like more details about how this was tested in ELISA, was it used in a sandwich ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 31 2004

    Answer

    Thank you for your phone call. Ab7526 was tested in a sandwich ELISA. General Instructions for sandwich ELISA to quantify collagen in solution: 1. Coat the plate with unlabeled anti-collagen (type specific) at 10 mg/ml. 2. Use biotinylated anti-collagen (same specificity) at a concentration of 0.1 mg/ml. 3. Dissolve the collagen firstly in 0.5 M Sodium Acetate. 4. Dilute the collagen in 0.05 M Tris Cl, 1.0 M Sodium Chloride, pH. 7.4. 5. Perform serial dilutions of the collagen using the Tris/NaCl buffer. 6. Perform all steps at 4C using overnight incubations. 7. All buffers are to be at 4C. 8. Use Streptavidin-HRP for detection.

    Read More

    Abcam Scientific Support

    Answered on Mar 31 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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