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    native-cow-collagen-iv-protein-ab7529.pdf

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Signal Transduction Cytoskeleton / ECM Basal Lamina
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Native Cow Collagen IV protein (ab7529)

  • Datasheet
  • SDS
Submit a review Q&A (2)

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Key features and details

  • Expression system: Native
  • Suitable for: WB, IP, ELISA

Description

  • Product name

    Native Cow Collagen IV protein
    See all Collagen IV proteins and peptides
  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Cow
    • Predicted molecular weight

      161 kDa

Specifications

Our Abpromise guarantee covers the use of ab7529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Immunoprecipitation

    ELISA

  • Form

    Liquid
  • Additional notes

    This protein is isolated from bovine placenta. 

    This product reacts with anti-Collagen Type IV. Reaction with anti-Collagen I, II, III, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituent: 0.82% Sodium acetate

General Info

  • Alternative names

    • Arresten
    • BSVD
    • CO4A1_HUMAN
    • COL4A1
    • COL4A1 NC1 domain
    • COL4A2
    • COL4A3
    • COL4A4
    • COL4A5
    • collagen alpha-1(IV) chain
    • Collagen IV Alpha 1 Polypeptide
    • Collagen IV Alpha 2 Polypeptide
    • Collagen Of Basement Membrane Alpha 1 Chain
    • Collagen Of Basement Membrane Alpha 2 Chain
    • Collagen Type IV Alpha 1
    • collagen type IV alpha 1 chain
    • Collagen Type IV Alpha 2
    • Collagen Type IV Alpha 3
    • Collagen Type IV Alpha 4
    • Collagen Type IV Alpha 5
    • RATOR
    see all
  • Function

    Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
    Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.
  • Tissue specificity

    Highly expressed in placenta.
  • Involvement in disease

    Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
    Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
    Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.
  • Sequence similarities

    Belongs to the type IV collagen family.
    Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.
  • Domain

    Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.
  • Post-translational
    modifications

    Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
    Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
    Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
    The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
    Proteolytic processing produces the C-terminal NC1 peptide, arresten.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Target information above from: UniProt accession P02462 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab7529? Please let us know so that we can cite the reference in this datasheet.

ab7529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

I would like to know where this type IV collagen (7536) comes from? Is it purified from human placenta? The info only states that it is native. Furthermore, do you know which alpha chains are present in this product?

Read More

Abcam community

Verified customer

Asked on Mar 20 2013

Answer

The Collagen IV protein (ab7529) has been isolated from bovine placenta. This contains both alpha chains of a1(IV) and a2(IV).

Read More

Abcam Scientific Support

Answered on Mar 20 2013

Question

Inquiry: Dear Sirs, We bought an aliquote of AB7529 - collagen type IV protein - for cleaving with different proteases. After MS analysis we find a few sequences in the alpha 3 chain of collagen type IV. Do you think we can trust these results? Do you know which alpha chains are in your isolated CO4? We would not expect there to be CO4-alpha3 in the human placenta. Best regards,

Read More

Abcam community

Verified customer

Asked on Jul 03 2012

Answer

Thank you for your enquiry and your interest in our products.

This protein (ab7529: Collagen IV protein) has been tested and characterized for WB, IP and ELISA. Although, the protein is in its native form (dissolved in 0.1M Sodium Acetate. pH 4.5) it is not known if it has any functional activity.

If you need anything further or any help then please let me know.

Read More

Abcam Scientific Support

Answered on Jul 03 2012

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