Native cow Protein C (ab81581)
- Datasheet
- References (1)
- Protocols
Description
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Product name
Native cow Protein C
See all Protein C proteins and peptides -
Biological activity
<1% APC activity. Assay is performed to determine percent contaminating APC activity. APC activity is based on a APC standard curve. Chromagenic assay uses Spectrozyme-TH as substrate.
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Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Cow
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Specifications
Our Abpromise guarantee covers the use of ab81581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 0.476% HEPES, 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Activation peptide
- Anticoagulant protein C
- APC
see all -
Function
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845). -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Thrombophilia due to protein C deficiency, autosomal dominant
Thrombophilia due to protein C deficiency, autosomal recessive -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide. -
Cellular localization
Secreted. Golgi apparatus. Endoplasmic reticulum. - Information by UniProt
Datasheets and documents
References
This product has been referenced in:
- Xiang T et al. Vasculogenic mimicry formation in EBV-associated epithelial malignancies. Nat Commun 9:5009 (2018). Read more (PubMed: 30479336) »
