Native Cow Proteoglycans from joint cartilage extract protein (ab134544)
Key features and details
- Expression system: Native
- Suitable for: Functional Studies
Description
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Product name
Native Cow Proteoglycans from joint cartilage extract protein -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Cow -
Additional sequence information
Source = Bovine articulate cartilage
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Specifications
Our Abpromise guarantee covers the use of ab134544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
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Form
Lyophilized -
Additional notes
ab134544 contains large proteoglycans consisting of core proteins with attached glycosaminoglycans. Major components of the mixture are aggrecan and versican, which are hyaluronic acid-binding proteoglycans. Both contain hyaluronic acid binding regions, Ig-like domains, EGF-like domains and lectin-like domains. As macromolecular polyanions they expand and hydrate the ECM in cartilage, intervertebral discs, dermis, blood vessel walls and brain tissue. Aggrecan and versican are degraded by proteases of the ADAMTS family. Aggrecan is cleaved by ADAMTS 1, 4, 5, 8, 9 and 15. Versican is hydrolyzed by ADAMTS 1, 4 and 9. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
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ReconstitutionSolubilize 150 mM Potassium Acetate pH 6.3 or PBS with at least 150 mM NaCl. The solution must stay till solubilization that can take 1-3 days, by 4-5°C to avoid bacterial contamination. We recommend to vortex the mixture from time to time during the day. The final conc of the solution should not exceed 1 mg/mL.
General Info
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Alternative names
- aggrecan
- cartilage proteoglycans
- large proteoglycans
- versican
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab134544 has not yet been referenced specifically in any publications.