Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Product nameNative cow Trypsin protein
See all Trypsin proteins and peptides
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab229834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immobilized Trypsin Digestion Protocol
1. Make a digestion buffer consisting of 0.1M Ammonium Bicarbonate, pH 8.0 (or other suitable buffer such as 0.1M Triethylamine Acetate, pH 8.0).
2. Dissolve 1 mg of your protein sample in 0.5ml digestion buffer.
3. Wash 0.15-0.3ml of immobilized trypsin with 3x0.5ml of digestion buffer. Separate the gel from the buffer after each wash by centrifugation or by column. Discard buffer after each wash.
4. Resuspend the washed immobilized trypsin gel in ~0.2ml of digestion buffer then add the resin to your protein sample.
5. Incubate mixture in a shaking water bath for 2-18 hours at 37C with rapid agitation.
6. Separate the immobilized trypsin gel from the digestion mixture as noted in step 3. Retain supernatant as your trypsin-digested protein sample.
Note: Optimization of immobilized trypsin protocol is required for specific applications. Recommended reaction conditions are pH 7.5 to 9.0 at 37C. The reaction rate will be increased by increasing the enzyme to protein substrate ratio and incubation temperature. For example, a typical digestion of 4 hours to overnight can be achieved using a ratio of 1:25 enzyme to protein substrate, while it is recommended to use 1:10 enzyme to protein substrate for accelerated trypsin digestion (30-60 minutes).
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.02% Sodium azide
Constituent: 50% Glycerol (glycerin, glycerine)
10 ml of 1:1 slurry
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Alpha-trypsin chain 2
- Anionic trypsinogen
- Beta trypsin
FunctionHas activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.
Involvement in diseaseDefects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229834 has not yet been referenced specifically in any publications.