Native Diphtheria Toxin (mutated G52E) protein (ab188505)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: WB, ELISA, Functional Studies, SDS-PAGE
Description
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Product name
Native Diphtheria Toxin (mutated G52E) protein -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Corynebacterium diphtheriae -
Predicted molecular weight
58 kDa -
Amino acids
1 to 536 -
Additional sequence information
CRM197 non-toxic mutant (52nd Gly to Glu ) was highly purified from growth media of the Corynebacterium diphtheriae mutant as mostly unnicked form.
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Specifications
Our Abpromise guarantee covers the use of ab188505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
CRM197 is a nontoxic mutant of Diphtheria toxin (Corynebacterium diphtheriae).
This is mutant diphtheria toxin which can not kill cells but can bind the receptor molecule (HB-EGF receptor) on animal cells.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 6
Constituents: 0.32% Tris HCl, 0.87% Sodium chloride
General Info
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Alternative names
- Corynebacterium diphtheriae toxoid
- Diphtheria Toxin
- tox
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Relevance
Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. -
Cellular localization
Secreted
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab188505 has been referenced in 2 publications.
- Catalioto RM et al. Booster immunization with a fractional dose of Prevnar 13 affects cell-mediated immune response but not humoral immunity in CD-1 mice. Heliyon 7:e07314 (2021). PubMed: 34195422
- Xiao Y et al. A Novel Interacting Protein SERP1 Regulates the N-Linked Glycosylation and Function of GLP-1 Receptor in the Liver. J Cell Biochem 118:3616-3626 (2017). PubMed: 28597972