Key features and details
- Expression system: Escherichia coli
- Active: Yes
Product nameNative E. coli beta Galactosidase protein (Active)
See all beta Galactosidase proteins and peptides
One Unit hydrolyzes one micromole of o-nitrophenyl-β-D-galactopyranoside per minute at 25°C, pH 7.5.
Activity: ≥50 units per mg dry weight.
Optimum pH range of 6 to 8.
Expression systemEscherichia coli
Protein lengthFull length protein
Predicted molecular weight540 kDa
Additional sequence informationBeta Galactosidase is a homotetramer with a molecular weight of 465.4 kDa (116.3 kDa subunits each).
Our Abpromise guarantee covers the use of ab7981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Extinction coefficient: = 20.9.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituent: 90% Galactosidase, beta
Optimal pH: 6-8
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in 0.1 M Sodium/potassium phosphate, pH 7.0.
- Beta D galactosidase
- Beta gal
- Beta galactosidase
RelevanceBeta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
ab7981 has been referenced in 1 publication.
- Lang D et al. Highly Variable Expression of CYP1A1 in Human Liver and Impact on Pharmacokinetics of Riociguat and Granisetron in Humans. Chem Res Toxicol 32:1115-1122 (2019). PubMed: 30950278