Native Human alpha 1 Antitrypsin protein (ab77937)

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Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Suitable for: SDS-PAGE

Description

Specifications

Our Abpromise guarantee covers the use of ab77937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

    Constituents: 0.492% Sodium phosphate, 1.74% Sodium chloride

  • Reconstitution
    Reconstitute with distilled water. Concentration is lot specific.

General Info

  • Alternative names

    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO0684
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all

  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).

  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.

  • Involvement in disease

    Alpha-1-antitrypsin deficiency

  • Sequence similarities

    Belongs to the serpin family.

  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.

  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab77937? Please let us know so that we can cite the reference in this datasheet.

ab77937 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-4 of 4 Q&A

Question

Inquiry: Hi, I am looking for a purified Alpha-1-antitrypsin protein to use for spiking samples in an ELISA (I am trying to validate your ab108799 ELISA kit for sputum samples). However, the A1AT standard provided in the ELISA kit is too dilute for this purpose. Can I ask what the solubility of your purified A1AT proteins is (particularly ab91136), or what the highest concentration you have been able to make of these proteins is? Best wishes,

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Answer

Thank you for your message.

I can confirm that all three of the Alpha 1 anti trypsin proteins in our catalog are from human, and are full length. Therefore, theoretically, they could be detected by the antibodies in the kit and be used for your experiments. However, please note they have not been tested in ELISA or with this kit, so regrettably we are not able to guarantee them for this purpose.

ab91136 alpha 1 Antitrypsin protein (Active)
Tested applications: SDS-PAGE, WB, FuncS
https://www.abcam.com/index.html?datasheet=91136 (or use the following: https://www.abcam.com/index.html?datasheet=91136).

ab77937 alpha 1 Antitrypsin protein (Human)
Tested applications: SDS-PAGE
https://www.abcam.com/index.html?datasheet=77937 (or use the following: https://www.abcam.com/index.html?datasheet=77937).

ab79181 alpha 1 Antitrypsin protein (Human)
Tested applications: SDS-PAGE
https://www.abcam.com/index.html?datasheet=79181 (or use the following: https://www.abcam.com/index.html?datasheet=79181).

I am sorry we have no data to confirm these would work in this case. However, I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

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Question

Thanks for the reply. I would like to confirm with you that both ab77937 and ab91136 are glycoproteins (glycosylated proteins) that purified from human plasma? I want to make sure that the proteins I am getting is glycosylated. Thank you. Best Regards,

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Answer

I can confirm that the ab77937 is also glycosylated. I hope tthis information will be helpful.

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Question

Thanks for the reply. I would like to confirm with you that both ab77937 and ab91136 are glycoproteins (glycosylated proteins) that purified from human plasma? I want to make sure that the proteins I am getting is glycosylated. Thank you. Best Regards,

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Answer

Thank you for your email. ab91136 is indeed glycosylated. I have asked our lab for confirmaiton about ab77937 also; I will forward you the information soon. I hope this will help.

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Question

Hi I would like to ask if your company carries any A1AT and UPK3A glycoproteins (I need glycosylated form). I noticed that you guys have ab77937 and ab91136 for A1AT, but may I know what is the difference between the two? According to your website, ab91136 is active form of A1AT, what is the meaning of active? Thanks and looking forward to hearing from you at your earliest possible time. Best Regards,  

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Answer

Thank you for contacting us. The only difference in ab91136 and ab77937 is that ab91136 is active form of protein; which means this protein is fully guaranteed to be used in functional assays. We unfortunately do not have products related to U3PKA protein. I would recommend searching in Biocompare website ( www.biocompare.com). They have very nice product search facility which will help. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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