Description

  • Product name

    Native human alpha 1 Antitrypsin protein (Active)
    See all alpha 1 Antitrypsin proteins and peptides
  • Biological activity

    When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inihibitory.

  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      52 kDa

Specifications

Our Abpromise guarantee covers the use of ab91136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.433

    Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 6.50
    Constituents: 0.492% Sodium phosphate, 1.74% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Reconstitute with distilled water. Once reconstituted, ab91136 is stable for one week at 4°C.

General Info

  • Alternative names

    • A1A
    • A1AT
    • A1AT_HUMAN
    • AAT
    • Alpha 1 antiproteinase
    • Alpha 1 antitrypsin
    • Alpha 1 antitrypsin null
    • Alpha 1 protease inhibitor
    • Alpha-1 protease inhibitor
    • Alpha-1-antiproteinase
    • alpha1 proteinase inhibitor
    • Alpha1AT
    • Dom1
    • PI
    • PI1
    • PRO0684
    • PRO2275
    • Serine (or cysteine) proteinase inhibitor clade A member 1
    • Serine protease inhibitor 1-1
    • Serine protease inhibitor A1a
    • Serpin A1
    • Serpin A1a
    • Serpin peptidase inhibitor clade A member 1
    • Serpina1
    • Short peptide from AAT
    • SPAAT
    • Spi1-1
    see all
  • Function

    Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
    Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE).
  • Tissue specificity

    Ubiquitous. Expressed in leukocytes and plasma.
  • Involvement in disease

    Alpha-1-antitrypsin deficiency
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Post-translational
    modifications

    N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
    Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418.
  • Cellular localization

    Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix.
  • Information by UniProt

Images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel


    Lane 1. 5 μg ab91136 (reduced/heated)
    Lane 2. 10 μg ab91136 (reduced/heated)
    Lane 3. 20 μg ab91136 (reduced/heated)
    Lane 4. Molecular weight markers
    Lane 5. 5 μg ab91136 (non-reduced/no heat)
    Lane 6. 10 μg ab91136 (non-reduced/no heat)
    Lane 7. 20 μg ab91136 (non-reduced/no heat)

References

This product has been referenced in:

  • Muley MM  et al. Prophylactic inhibition of neutrophil elastase prevents the development of chronic neuropathic pain in osteoarthritic mice. J Neuroinflammation 14:168 (2017). Read more (PubMed: 28835277) »
See 1 Publication for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

The protein concentration of lot GR 108058 was 3.79 mg/ml. Each mg was lyophilized from 264 ul of buffer and you should reconstitute with 264 ul of deionized water in order to maintain a buffer of 30 mM Na Phosphate and 300 mM NaCl.

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Answer

Thank you for your enquiry.

We do not have set expiration dates for our products. Most proteins are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage instructions on the datasheet for the product you purchased. These conditions will vary among our products, therefore, it is important to verify the storage conditions for each of our products when you receive them. We guarantee all of our products to work for at least 120 days from the date of purchase when stored correctly. For more information on this guarentee please refer to the following link:

www.abcam.com/abpromise

With respect to Alpha 1 Antitrypsin (ab91136) in particular we expect the product to be stable, lyophilized at -20°C, for five years.

Reconstituted, we expect the product to be stable for about one week on ice, or stored at 4°C.

I hope this information helps. Please do not hesitate to contact us if you need anything further.

Read More

Answer

Thank you for your reply.

Unfortunately we only have the testing information for the porcine pancreatic trypsin. It may work with human or bovine, but we just don't know for sure since this hasn't been tested.

I apologize that this information could not be more helpful. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

When ab91136 was tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inihibitory.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Vielen Dank für Ihren Anruf.

Das lyophilisierte Protein sollte bei -20C gelagert werden. Wenn das Protein mit deionisierten Wasser resuspendiert wurde, lagern Sie es bitte in Aliquotesbei -80C.

Der EC ist übrigensfür jedes Protein ist einzigartig. Das heißt natürlich nicht, dasszwei Proteine nicht den gleichen EC haben können

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

The original concentration of the lot you received was 2.3 mg/ml, or 1 mg in 434.8 ul.

So, adding 434.8 ul of de-ionized water will reconstitute the constituents to their original concentrations and pH: 300mM Sodium chloride, 30mM Sodium phosphate, pH 6.5.

Please do not hesitate to contact us if you need any more advice or information.

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Answer

I can confirm that the ab77937 is also glycosylated. I hope tthis information will be helpful.

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Answer

Thank you for your email. ab91136 is indeed glycosylated. I have asked our lab for confirmaiton about ab77937 also; I will forward you the information soon. I hope this will help.

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Answer

Thank you for contacting us. The only difference in ab91136 and ab77937 is that ab91136 is active form of protein; which means this protein is fully guaranteed to be used in functional assays. We unfortunately do not have products related to U3PKA protein. I would recommend searching in Biocompare website ( www.biocompare.com). They have very nice product search facility which will help. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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