Key features and details
- Expression system: Native
- Suitable for: RID, SDS-PAGE
Product nameNative Human Alpha 1 microglobulin protein
See all Alpha 1 microglobulin proteins and peptides
Protein lengthFull length protein
Predicted molecular weight30 kDa
Amino acids20 to 203
Our Abpromise guarantee covers the use of ab96148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesStarting material tested and found negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C.
Constituent: 0.158% Ammonium bicarbonate
May contain traces of buffer salts
ReconstitutionReconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.
- Alpha 1 microglobulin/bikunin precursor
- Alpha 1 microglycoprotein
FunctionInter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
Trypstatin is a trypsin inhibitor.
Tissue specificityExpressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
Sequence similaritiesIn the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
Contains 2 BPTI/Kunitz inhibitor domains.
modificationsThe precursor is proteolytically processed into separately functioning proteins.
3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab96148 has not yet been referenced specifically in any publications.