Key features and details
- Expression system: Mammalian
- Purity: >= 95% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameNative Human alpha 2 Macroglobulin protein
See all alpha 2 Macroglobulin proteins and peptides
Purity>= 95 % SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight180 kDa
Molecular weight information725 kDa (Homotetramer, Subunit size: 180 kDa)
Additional sequence informationSource: Human Plasma. Shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for antiHIV 1 & 2 by FDA approved tests.
Our Abpromise guarantee covers the use of ab285822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 7297 Alpha 2 Macroglobulin, Human Plasma, Fast Form. 7297-1000 is the same size as the 1 mg size of ab285822.
Alpha 2 Macroglobulin (A2M) is a plasma protease inhibitor which has been shown to exist in two forms. The Slow Form of A2M (S-A2M) is the form which possesses the ability to bind and inhibit proteases by a “trap” method. The Fast Form of A2M (F-A2M) is generated when S-A2M undergoes a conformational change due to either entrapment of a protease in the A2M bait region, or chemical cleavage of an internal thiol ester bond located near the bait region. F-A2M does not possess the ability to bind and inhibit protease activity. F-A2M is rapidly taken up by the liver, with a half-life of 2-4 minutes. In vivo, F-A2M typically represents only 0.17–0.7% of the total A2M in blood plasma of adults. The F-A2M plasma concentration is, however, increased in many disease states including pancreatitis, multiple sclerosis and sepsis. F-A2M has also been implicated in the inhibition of amyloid formation associated with Alzheimer’s disease and spongiform encephalopathy.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 1.639% Sodium phosphate
ReconstitutionIn water or aqueous buffer
- Alpha 2 M
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
- Information by UniProt
4-20% Novex Tris-Glycine Gel with 1X Tris-glycine running buffer
1. ab285822 - 2 µg (no heat/reduced)
2. ab285822 - 4 µg (no heat/reduced)
3. ab285822 - 8 µg (no heat/reduced)
4. ab285822 - 10 µg (no heat/reduced)
6. ProSieve Color Protein Markers
7. ab285822 - 2 µg (heated/reduced)
8. ab285822 - 4 µg (heated/reduced)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab285822 has not yet been referenced specifically in any publications.