Native Human alpha 2 Macroglobulin protein (ab91104)
- Datasheet
- References
- Protocols
Description
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Product name
Native Human alpha 2 Macroglobulin protein
See all alpha 2 Macroglobulin proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilised -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.81. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20ºC.
pH: 7.00
Constituents: Glycine, 0.492% Sodium phosphate
General Info
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Alternative names
- A2m
- A2MG_HUMAN
- Alpha 2 M
see all -
Function
Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. -
Tissue specificity
Secreted in plasma. -
Sequence similarities
Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. -
Developmental stage
Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Native Human alpha 2 Macroglobulin protein (ab91104)SDS-PAGE: 4-12% Bis-Tris-HCl NuPAGE gel
Lane 1. 5 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 2. 10 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 3. 20 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 4. 5 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 5. 10 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 6. 20 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 7. Molecular weight markers
Datasheets and documents
References
ab91104 has not yet been referenced specifically in any publications.
