Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameNative Human alpha 2 Macroglobulin protein
See all alpha 2 Macroglobulin proteins and peptides
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.81. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
Constituents: Glycine, 0.492% Sodium phosphate
- Alpha 2 M
FunctionIs able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
Tissue specificitySecreted in plasma.
Sequence similaritiesBelongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
Developmental stageContrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
- Information by UniProt
SDS-PAGE: 4-12% Bis-Tris-HCl NuPAGE gel
Lane 1. 5 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 2. 10 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 3. 20 μg alpha 2 Macroglobulin (non-reduced/no heat)
Lane 4. 5 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 5. 10 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 6. 20 μg alpha 2 Macroglobulin (reduced/ heated)
Lane 7. Molecular weight markers
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91104 has not yet been referenced specifically in any publications.