Description

Specifications

Our Abpromise guarantee covers the use of ab91104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Lyophilised
  • Additional notes

    Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.81. Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20ºC.

    pH: 7.00
    Constituents: Glycine, 0.492% Sodium phosphate

General Info

  • Alternative names

    • A2m
    • A2MG_HUMAN
    • Alpha 2 M
    • Alpha 2M
    • Alpha-2-M
    • Alpha-2-macroglobulin
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
    • CPAMD5
    • DKFZp779B086
    • FWP007
    • S863 7
    see all
  • Function

    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • Tissue specificity

    Secreted in plasma.
  • Sequence similarities

    Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.
  • Developmental stage

    Contrary to the rat protein, which is an acute phase protein, this protein is always present at high levels in circulation.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE: 4-12% Bis-Tris-HCl NuPAGE gel
    Lane 1. 5 μg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 2. 10 μg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 3. 20 μg alpha 2 Macroglobulin (non-reduced/no heat)
    Lane 4. 5 μg alpha 2 Macroglobulin (reduced/ heated)
    Lane 5. 10 μg alpha 2 Macroglobulin (reduced/ heated)
    Lane 6. 20 μg alpha 2 Macroglobulin (reduced/ heated)
    Lane 7. Molecular weight markers

References

ab91104 has not yet been referenced specifically in any publications.

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