Key features and details
- Expression system: Mammalian
- Purity: >= 95% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameNative Human Antithrombin III/ATIII protein
See all Antithrombin III/ATIII proteins and peptides
Purity>= 95 % SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight52 kDa
Actual molecular weight58 kDa
Additional sequence informationSource: Human Plasma. Shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for antiHIV 1 & 2 by FDA approved tests.
Our Abpromise guarantee covers the use of ab285823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 7298 Antithrombin III, Human Plasma. 7298-500 is the same size as the 500 µg size of ab285823.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: 0.788% Tris HCl, 0.877% Sodium chloride
ReconstitutionIn water or aqueous buffer
- Antithrombin III
FunctionMost important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Tissue specificityFound in plasma.
Involvement in diseaseDefects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
Sequence similaritiesBelongs to the serpin family.
modificationsPhosphorylation sites are present in the extracelllular medium.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
Antithrombin III, Human Plasma (ab285823)
4-12% Bis-Tris NuPAGE gel
1. ab285823 - 5 µg (non-reduced / no heat)
2. ab285823 - 10 µg (non-reduced / no heat)
3. ab285823 - 20 µg (non-reduced / no heat)
4. LMW Standard
5. ab285823 - 5 µg (reduced / heated)
6. ab285823 - 10 µg (reduced / heated)
7. ab285823 - 20 µg (reduced / heated)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab285823 has not yet been referenced specifically in any publications.