Native Human Antithrombin III/ATIII protein (ab95077)



Our Abpromise guarantee covers the use of ab95077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications



  • Form

  • Additional notes

    2ml of 1:1 slurry

    1.0 mg/ml coupled 

    May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
    1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
    2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
    3. Wash and elute heparin with 3M NaCl in TBS or PBS.
    4. Re-equilabrate resin in TBS or PBS.
    5. Add 0.02percent Sodium Azide for storage.

    Previously labelled as Antithrombin III.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 1.58% Tris HCl, 0.87% Sodium chloride

General Info

  • Alternative names

    • ANT3_HUMAN
    • Antithrombin
    • Antithrombin III
    • Antithrombin-III
    • AntithrombinIII
    • AT 3
    • AT III
    • AT3
    • AT3D
    • ATIII
    • Heparin cofactor I
    • MGC22579
    • Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1
    • Serine cysteine proteinase inhibitor clade C member 1
    • Serine proteinase inhibitor clade C member 1
    • Serpin C1
    • Serpin family C member 1
    • Serpin peptidase inhibitor clade C (antithrombin) member 1
    • SERPINC1
    • THPH7
    see all
  • Function

    Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Tissue specificity

    Found in plasma.
  • Involvement in disease

    Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational

    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt


ab95077 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your inquiry.

I cannot recommend ab81748 (Factor Xa protein) for your customer. I believe your customer is looking for human protein and this one is from cow.

Maybe your customer would like to have a look at this link to find more active Factor Xa proteins:

Concerning the Antithrombin III proteins: two of them are tested for their functionality:

ab77942 and ab91119 were tested for functionality by verifying thatthey will complex with Thrombin. Unfortunately, the assay does not measure “activity units”.

I hope this information is helpful for your customer. Please feel free to contact me again with any further questions.

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