Native Human Antithrombin III/ATIII protein (ab95077)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: Purification, SDS-PAGE
Description
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Product name
Native Human Antithrombin III/ATIII protein
See all Antithrombin III/ATIII proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Specifications
Our Abpromise guarantee covers the use of ab95077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Purification
SDS-PAGE
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Form
Liquid -
Additional notes
2ml of 1:1 slurry
1.0 mg/ml coupled
May be used repeatedly. Protocol for purifying high affinity heparin with immobilized antithrombin:
1. Equilibrate immobilized antithrombin in TBS (0.1M Tris-HCl, 0.15M NaCl, pH 7.4) or PBS (0.05M Sodium Phosphate, 0.15M NaCl, pH 7.4).
2. Apply heparin in TBS or PBS. Binding capacity is ~0.1 mg high affinity heparin / ml resin and must be determined by the end user.
3. Wash and elute heparin with 3M NaCl in TBS or PBS.
4. Re-equilabrate resin in TBS or PBS.
5. Add 0.02percent Sodium Azide for storage.Previously labelled as Antithrombin III.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C.
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1.58% Tris HCl, 0.87% Sodium chloride
General Info
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Alternative names
- ANT3_HUMAN
- Antithrombin
- Antithrombin III
see all -
Function
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Tissue specificity
Found in plasma. -
Involvement in disease
Defects in SERPINC1 are the cause of antithrombin III deficiency (AT3D) [MIM:613118]. AT3D is an important risk factor for hereditary thrombophilia, a hemostatic disorder characterized by a tendency to recurrent thrombosis. AT3D is classified into 4 types. Type I: characterized by a 50% decrease in antigenic and functional levels. Type II: has defects affecting the thrombin-binding domain. Type III: alteration of the heparin-binding domain. Plasma AT-III antigen levels are normal in type II and III. Type IV: consists of miscellaneous group of unclassifiable mutations. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsPhosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab95077 has not yet been referenced specifically in any publications.