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Kindly provide additional information about the conditions of WB that is shown in the image in the datasheet.
Asked on Nov 06 2012
Thank you for contacting us.
I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentration the lane was overloaded.
The membrane was blocked blocked for 1 hour at room temperature with 5% BSA in 0.1% TBST, with gentle rocking. Primary antibody was then diluted (1 µg/ml) with 5% BSA in 0.1% TBST and incubated overnight at 4°C with gentle rocking. The membrane was washed and incubated with Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 diluted with 5% BSA in 0.1% TBST with gentle rocking for 1 hour.
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Answered on Nov 06 2012