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    native-human-azurocidin-protein-ab91129.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Native Human Azurocidin protein (ab91129)

  • Datasheet
  • SDS
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Western blot - Native Human Azurocidin protein (ab91129)
  • SDS-PAGE - Native Human Azurocidin protein (ab91129)

Key features and details

  • Expression system: Native
  • Purity: > 95% SDS-PAGE
  • Suitable for: WB, SDS-PAGE

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ELISA
Product image
Human Azurocidin ELISA Kit (ab213755)

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Description

  • Product name

    Native Human Azurocidin protein
    See all Azurocidin proteins and peptides
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human

Associated products

  • Related Products

    • Anti-Azurocidin antibody (ab48132)
    • Biotin Anti-Azurocidin antibody (ab51508)
    • Anti-Azurocidin antibody (ab88819)

Specifications

Our Abpromise guarantee covers the use of ab91129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Lyophilized
  • Additional notes

    Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

General Info

  • Alternative names

    • Azu 1
    • AZU1
    • Azurocidin
    • CAP37
    • CAP7_HUMAN
    • Cationic antimicrobial protein 37
    • Cationic antimicrobial protein CAP37
    • HBP
    • Heparin binding protein
    • Heparin-binding protein
    • HUMAZUR
    • Neutrophil azurocidin
    see all
  • Function

    This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.
  • Sequence similarities

    Belongs to the peptidase S1 family. Elastase subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Cytoplasmic granule. Cytoplasmic granules of neutrophils.
  • Target information above from: UniProt accession P20160 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Western blot - Native Human Azurocidin protein (ab91129)
    Western blot - Native Human Azurocidin protein (ab91129)
  • SDS-PAGE - Native Human Azurocidin protein (ab91129)
    SDS-PAGE - Native Human Azurocidin protein (ab91129)
    Lane 1. LMW Standard
    Lane 2. Azurocidin - 5 μg (reduced / heated)
    Lane 3. Azurocidin - 10 μg (reduced / heated)
    Lane 4. Azurocidin - 20 μg (reduced / heated)
    4-12% Bis-Tris SDS-PAGE gel

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab91129? Please let us know so that we can cite the reference in this datasheet.

ab91129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Dear Sir/Madam, I have ordered Human Azurocidin protein (Ab91129) which arrived yesterday. Would you please let me know which buffer is this protein lyophilised from? Thanks  

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Abcam community

Verified customer

Asked on Sep 28 2011

Answer

Thank you for contacting us. This protein is lyophilised from de-ionized water, and reconstituted to pre-lyophilisation volume with de-ionized water. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 28 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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