Overview

  • Product name

    Native Human C1q protein
  • Protein length

    Full length protein

Description

  • Nature

    Native
  • Source

    Native
  • Amino Acid Sequence
    • Species

      Human
    • Molecular weight

      410 kDa

Specifications

Our Abpromise guarantee covers the use of ab96363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Immunodiffusion

  • Purity

    >= 96 % SDS-PAGE.
    Nominally greater than 96% of the protein is C1q
  • Form

    Lyophilised
  • Additional notes

    Starting material serum donor tested and certified negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C.

    pH: 7.50
    Constituents: 1.74% Sodium chloride, 0.29% Sodium EDTA

  • Reconstitution
    Determine the volume of water required to reconstitute the protein based on the concentration given. Slight turbidity may result on reconstitution.

General Info

  • Alternative names

    • C1qa
    • C1QA_HUMAN
    • C1QB
    • C1QC
    • Complement C1q subcomponent subunit A
    • Complement C1q subcomponent subunit B
    • Complement C1q subcomponent subunit C
    • Complement component 1 q subcomponent alpha polypeptide
    • Complement component C1q A chain
    • Complement component C1q B chain
    • Complement component C1q C chain
    see all
  • Function

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
  • Involvement in disease

    Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.
  • Sequence similarities

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.
  • Post-translational
    modifications

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.
  • Cellular localization

    Secreted.
  • Information by UniProt

References

This product has been referenced in:

  • Zhou W  et al. Characterization of antibody-C1q interactions by Biolayer Interferometry. Anal Biochem 549:143-148 (2018). Read more (PubMed: 29577875) »
  • Minutti CM  et al. Local amplifiers of IL-4Ra-mediated macrophage activation promote repair in lung and liver. Science 356:1076-1080 (2017). Read more (PubMed: 28495878) »
See all 3 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

Thank you for your reply.

I checked with the lab and we have not tested the protein for stability after reconstitution and would advise to immediately use the protein after reconstitution.

The buffer does not contain any protease inhibitor or stabilisers.

I hope this information is helpful and wish you good luck with your research.

Read More

Question
Answer

Thank you very much for your patience.

I can now confirm that we recommend to reconstitute lot GR116211-1 in 700ul ddH2O.

This will provide a concentration of ca. 1.43mg/ml.

I hope this information is helpful and wish you good luck with your research and a good weekend.

Read More

Answer

Indeed, this sounds a bit confusing. However, in our internal notes i found that this volume is in relation to the original concentration of the lot which is used. Do you mind sending me the lot. number you received so I can check the concentration for you?

Read More

Answer

Thank you for contacting regarding purified mouse C1q.
Unfortunately we are unable to offer this product for sale at this time. As I had mentioned in previous correspondences we do have a purified human protein and mouse peptide available should you be interested in using those:

Human protein (ab96363): https://www.abcam.com/c1q-protein-ab96363.html
Mouse peptide (ab91203): https://www.abcam.com/C1q-peptide-ab91203.html

Another option may be to use Mouse complement serum. While we do not offer this you should be able to find it by searching at www.biocompare.com

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

I would be glad to see if we may be able to place this product on our catalog for you and have contacted the lab to determine this. It may take a few weeks to add this product and be to ship.

We currently have the purified human C1q protein (ab96363) and a synthetic mouse C1q peptide derived from within residues 50 - 150 of (ab91203).This peptide was used as an immunogen for ab64632 - C1q antibody. Both of these products are available to ship now.

I hope this information is helpful to you and will contact you should I receive more information. Please do not hesitate to contact us if you need any more advice or information.



Use our products? Submit an Abreview. Earn rewards!

https://www.abcam.com/abreviews

Read More

Question
Answer

Thank you for your patience, while I contacted the lab about the reconstitution volume.

The reconstitution volume before lyophilisation was 5.9mls. I am double checking with them, whether this is the actual volume they recommend to reconstitute in. Hopefully I will be able to get to you much quicker this time.

Once gain, I appreciate your patience in this matter.

Read More

Question
Answer

Thank you for contacting Abcam yesterday.

I just wanted to let you know that I am still waiting to hear back from the lab about the volume to reconstitute the protein and as soon as I hear anything from them, I will forward it on.

Thank you for your patience in this matter.

Read More

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