Overview

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Molecular weight
      110 kDa

Specifications

Our Abpromise guarantee covers the use of ab91019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    >=1,000 units per mg protein.

    One unit is defined as the amount of enzyme that hydrolyzes one picomole of Suc-LLVY-AMC per minute at 25degC.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 95 % SDS-PAGE.

  • Form
    Liquid
  • Additional notes

    Prepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

    Protein determination: Bradford Assay.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 6.80
    Preservative: 0.14% Imidazole
    Constituents: 0.038% EGTA, 0.039% Beta mercaptoethanol, 0.0292% EDTA, 30% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • Ca2 activated neutral protease
    • Calcium activated neutral proteinase
    • Calcium activated neutral proteinase small subunit
    • Calcium dependent protease small subunit
    • Calcium dependent protease small subunit 1
    • Calcium-activated neutral proteinase 1
    • Calpain 1
    • Calpain 1 large subunit
    • Calpain 1, (mu/I) large subunit
    • Calpain mu type
    • Calpain mu-type
    • Calpain regulatory subunit
    • Calpain small subunit 1
    • Calpain, large polypeptide L1
    • Calpain-1 catalytic subunit
    • Calpain-1 large subunit
    • CAN1_HUMAN
    • CANP
    • CANP 1
    • CANP small subunit
    • CANP1
    • CANPL 1
    • CANPL1
    • CAPN 1
    • CAPN1
    • Cell proliferation inducing protein 30
    • Cell proliferation-inducing gene 30 protein
    • Micromolar Calpain
    • Micromolar-calpain
    • Mu Calpain
    • muCANP
    • muCL
    see all
  • Function
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • Cellular localization
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt

Images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel


    Lane 1: 5 μg ab91019 (reduced /heated). 
    Lane 2: 10 μg ab91019 (reduced/ heated). 
    Lane 3: Molecular weight markers.
    Lane 4: 5 μg ab91019 (non-reduced/no heat). 
    Lane 5: 10 μg ab91019 (non-reduced/no heat).

  • Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Native human Calpain 1 protein (Active) (ab91019) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

References

This product has been referenced in:
  • Wolhuter K  et al. Evidence against Stable Protein S-Nitrosylation as a Widespread Mechanism of Post-translational Regulation. Mol Cell 69:438-450.e5 (2018). Read more (PubMed: 29358077) »
  • Hossain MJ  et al. The Soluble Form of Toll-Like Receptor 2 Is Elevated in Serum of Multiple Sclerosis Patients: A Novel Potential Disease Biomarker. Front Immunol 9:457 (2018). Read more (PubMed: 29593720) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

The specific activity for GR23778 is 2126 Units/mg.

Read More

Answer

Hello. No, this is not exactly the same, the protein ab91019 comes from another source. They are very similar though. Please let me know if you have any further questions.

Read More

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