Product nameNative human Calpain 1 protein (Active)
See all Calpain 1 proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence
Molecular weight110 kDa
Our Abpromise guarantee covers the use of ab91019 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>=1,000 units per mg protein.
One unit is defined as the amount of enzyme that hydrolyzes one picomole of Suc-LLVY-AMC per minute at 25degC.
Purity> 95 % SDS-PAGE.
Prepared from whole blood shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Protein determination: Bradford Assay.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.14% Imidazole
Constituents: 0.038% EGTA, 0.039% Beta mercaptoethanol, 0.0292% EDTA, 30% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Ca2 activated neutral protease
- Calcium activated neutral proteinase
- Calcium activated neutral proteinase small subunit
FunctionCalcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Sequence similaritiesBelongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cellular localizationCytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
- Information by UniProt
SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg ab91019 (reduced /heated).
Lane 2: 10 μg ab91019 (reduced/ heated).
Lane 3: Molecular weight markers.
Lane 4: 5 μg ab91019 (non-reduced/no heat).
Lane 5: 10 μg ab91019 (non-reduced/no heat).
This product has been referenced in:
- Wolhuter K et al. Evidence against Stable Protein S-Nitrosylation as a Widespread Mechanism of Post-translational Regulation. Mol Cell 69:438-450.e5 (2018). Read more (PubMed: 29358077) »
- Hossain MJ et al. The Soluble Form of Toll-Like Receptor 2 Is Elevated in Serum of Multiple Sclerosis Patients: A Novel Potential Disease Biomarker. Front Immunol 9:457 (2018). Read more (PubMed: 29593720) »