For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
One our customers wants to purchase antibodies to test in sELISA and human samples. They want to detect cardiac troponins T and I. 1) anti-cardiac troponin T : ab45932 to coat on surface, then the protein ab47005 and then the FITC conjugated antibody ab105439 for detection. 2) anti-cardiac troponin I : ab27972, then Cardiac Troponin I protein ab50803 and ab56357 that will be conjugated with FITC using the Easylink kit. Would it work for their sELISA experiments?
Asked on Sep 15 2011
Thank you for contacting us. I would recommend to use : 1) For the detection of Cardiac Troponin T : - ab45932 as capture antibody (directed against amino acids 64-82) www.abcam.com/ab45932 , - ab9937 as protein target, www.abcam.com/ab9937 , (not ab47005 which is the immunogen peptide used to raise ab45932), - ab105439 as detection antibody (directed against amino acids 64-82), www.abcam.com/ab105439 . 2) For the detection of Cardiac Troponin I : - ab115704 as capture antibody, www.abcam.com/ab115704 , - ab50803 as protein target, www.abcam.com/ab50803 , - ab19615 as detection antibody (can be conjugated with FITC using the EasyLink conjugation kit), www.abcam.com/ab19615 . I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Sep 15 2011