Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameNative human Catalase protein
See all Catalase proteins and peptides
Biological activityActivity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight230 kDa
Additional sequence informationPurified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.
Our Abpromise guarantee covers the use of ab91026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituent: 0.79% Tris HCl
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similaritiesBelongs to the catalase family.
modificationsThe N-terminus is blocked.
- Information by UniProt
SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Catalase (reduced/heated)
Lane 2: 10 μg Catalase (reduced/heated)
Lane 3: 20 μg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg Catalase (non-reduced/no heat)
Lane 6: 10 μg Catalase (non-reduced/no heat)
Lane 7: 20 μg Catalase (non-reduced/no heat)
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :
Native human Catalase protein (ab91026) at 0.1 µg
Lane 2 :
Native human Catalase protein (ab91026) at 0.01 µg
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91026 has been referenced in 1 publication.
- Czechowska E et al. Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces N/A:N/A (2018). PubMed: 30054048