Description

  • Product name

    Native human Catalase protein
    See all Catalase proteins and peptides
  • Biological activity

    Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
  • Purity

    > 95 % SDS-PAGE.

  • Expression system

    Native
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Native
    • Species

      Human
    • Predicted molecular weight

      230 kDa
    • Additional sequence information

      Purified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.

Specifications

Our Abpromise guarantee covers the use of ab91026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

    Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituent: 0.79% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Cas1
    • CAT
    • CATA_HUMAN
    • Catalase
    • Cs1
    • MGC138422
    • MGC138424
    see all
  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities

    Belongs to the catalase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Peroxisome.
  • Information by UniProt

Images

  • SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
    Lane 1: 5 μg Catalase (reduced/heated)
    Lane 2: 10 μg Catalase (reduced/heated)
    Lane 3: 20 μg Catalase (reduced/heated)
    Lane 4: Molecular weight markers
    Lane 5: 5 μg Catalase (non-reduced/no heat)
    Lane 6: 10 μg Catalase (non-reduced/no heat)
    Lane 7: 20 μg Catalase (non-reduced/no heat)
  • All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml

    Lane 1 : Native human Catalase protein (ab91026) at 0.1 µg
    Lane 2 : Native human Catalase protein (ab91026) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

References

This product has been referenced in:

  • Czechowska E  et al. Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces N/A:N/A (2018). Read more (PubMed: 30054048) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting Abcam.

Having talked to the lab, they told me that frozen Catalase has the appearancethat you described. Therefore the vial is not contaminated as I first thought andso you can continue your experiments as normal.

If there is anything elseI can help you with, pleaselet me know.

Read More

Answer

Thank you for contacting Abcam.

I am sorry that the vial of ab91026 was contaminated upon arrival. I have set the product to ship on Monday and it should arrive to you on Tuesday morning. Your new order number is ******.

Please let me know if there is anything else I can help you with.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up