Native human Catalase protein (ab91026)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Native human Catalase protein
See all Catalase proteins and peptides -
Biological activity
Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0 -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
230 kDa -
Additional sequence information
Purified from Human Erythrocytes. Four identical subunits each with a molecular weight of 58,000.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituent: 0.79% Tris HClThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Cas1
- CAT
- CATA_HUMAN
see all -
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
Images
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SDS-PAGE - Native human Catalase protein (ab91026)SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 μg Catalase (reduced/heated)
Lane 2: 10 μg Catalase (reduced/heated)
Lane 3: 20 μg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 μg Catalase (non-reduced/no heat)
Lane 6: 10 μg Catalase (non-reduced/no heat)
Lane 7: 20 μg Catalase (non-reduced/no heat) -
Western blot - Native human Catalase protein (ab91026)All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :Native human Catalase protein (ab91026) at 0.1 µg
Lane 2 :Native human Catalase protein (ab91026) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab91026 has been referenced in 1 publication.
- Czechowska E et al. Comparison of the antioxidant activity of catalase immobilized on gold nanoparticles via specific and non-specific adsorption. Colloids Surf B Biointerfaces N/A:N/A (2018). PubMed: 30054048