Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameNative human Cathepsin D protein
See all Cathepsin D proteins and peptides
Biological activityActivity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lyophilized from 2 mM Na phosphate, pH 6.5.
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.0 Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.
Constituent: 0.0328% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionResuspend a 25ug vial with 71.4uL of deionized H2O to give a protein concentration of 0.350mg/mL, which is the original protein concentration of the lot when it was finished. This should then be aliquotted into suitable amounts in a screw top vial to prevent sublimation and stored at <70 deg C. Aliquots should be diluted to appropriate concentration once thawed. We recommend 2mM NaP pH 6.5 to make dilutions.
- Cathepsin D
FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue specificityExpressed in the aorta extrcellular space (at protein level).
Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
Sequence similaritiesBelongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
modificationsN- and O-glycosylated.
Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1. Molecular weight markers
Lane 2. 4 μg Cathepsin D (non-reduced/no heat)
Lane 3. 7 μg Cathepsin D (non-reduced/no heat)
Lane 4. Molecular weight markers
Lane 5. 4 μg Cathepsin D (reduced/heated)
Lane 6. 7 μg Cathepsin D (reduced/heated)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91123 has been referenced in 1 publication.
- Brings S et al. Urinary cathepsin L is predictive of changes in albuminuria and correlates with glucosepane in patients with type 2 diabetes in a closed-cohort study. J Diabetes Complications N/A:107648 (2020). PubMed: 32532588