Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameNative human Cathepsin G protein
See all Cathepsin G proteins and peptides
Biological activityActivity: 2-4 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala Pro-Phe-pNA (1 mM) per minute at 25°C in 160 mM Tris-HCl, pH 7.4, with 1.6 M NaCl.
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab91122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Additional notesProtein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.66. Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti- HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20ºC.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Reconstitute in 150 mM sodium acetate (pH 5.5), 150 mM NaCl.
- Cathepsin G
FunctionSerine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Cellular localizationCell surface.
- Information by UniProt
SDS-PAGE: 12% Tris-HCl gel
Lane 1. 5 μg Cathepsin G (reduced/heated)
Lane 2. 10 μg Cathepsin G (reduced/heated)
Lane 3. 20 μg Cathepsin G (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg Cathepsin G (non-reduced/no heat)
Lane 6. 10 μg Cathepsin G (non-reduced/no heat)
Lane 7. 20 μg Cathepsin G (non-reduced/no heat)
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab91122 has not yet been referenced specifically in any publications.