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Native human CD13 protein (ab134454)

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SDS-PAGE - Native human CD13 protein (ab134454)

    Key features and details

    • Expression system: Native
    • Purity: > 90% SDS-PAGE
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE

    Description

    • Product name

      Native human CD13 protein
      See all CD13 proteins and peptides

    • Biological activity

      The active enzyme is inhibited by Amastatin hydrochloride, Actinonin and Bestatin hydrochloride (approx 150 µM). Specific activity of ab134454 is >500 mU/mg (L-Leu-pNA at 37°C, pH 7.2)

    • Purity

      > 90 % SDS-PAGE.

    • Expression system

      Native

    • Accession

      P15144

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Native

      • Species

        Human

      • Sequence

        MAKGFYISKSLGILGILLGVAAVCTIIALSVVYSQEKNKNANSSPVASTT PSASATTNPASATTLDQSKAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGL YVFKGSSTVRFTCKEATDVIIIHSKKLNYTLSQGHRVVLRGVGGSQPPDI DKTELVEPTEYLVVHLKGSLVKDSQYEMDSEFEGELADDLAGFYRSEYME GNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHP KDLTALSN MLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGV LIRIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPD FNAGAMENWGLVTYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNL VTIEWWNDLWLNEGFASYVEYLGADYAEPTWNLKDLMVLNDVYRVMAVDA LASSHPLSTPASEINTPAQISELFDAISYSKGASVLRMLSSFLSEDVFKQ GLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIMNRWTLQMG FPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQ DYWLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQR DHSAIPVINRAQ IINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWE AALSSLSYFKLMFDRSEVYGPMKN YLKKQVTPLFIHFRNNTNNWREIP ENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWM ENPNNNPIHPNL RSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWI LNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFND YGGGSFSFSN LIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALE QALEKTKANIKWVKENKEVVLQ WFTENSK

      • Predicted molecular weight

        110 kDa

      • Amino acids

        1 to 967

      • Additional sequence information

        Purified from human serum

    Specifications

    Our Abpromise guarantee covers the use of ab134454 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      ab134454 is certified to be negative for HBsAg and for antibodies to HIV and HCV.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.40
      Constituents: 0.01% Calcium chloride, 0.32% Tris HCl, 2.92% Sodium chloride

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    General Info

    • Alternative names

      • Alanyl (membrane) aminopeptidase
      • Alanyl aminopeptidase
      • Aminopeptidase M
      • Aminopeptidase N
      • AMPN_HUMAN
      • ANPEP
      • AP M
      • AP N
      • AP-M
      • AP-N
      • APN
      • CD 13
      • CD13
      • CD13 antigen
      • gp150
      • hAPN
      • LAP 1
      • LAP1
      • Microsomal aminopeptidase
      • Myeloid plasma membrane glycoprotein CD13
      • p150
      • PEPN
      see all

    • Function

      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

    • Tissue specificity

      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

    • Sequence similarities

      Belongs to the peptidase M1 family.

    • Domain

      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).

    • Post-translational
      modifications

      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.

    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Information by UniProt

    Images

    • SDS-PAGE - Native human CD13 protein (ab134454)
      SDS-PAGE - Native human CD13 protein (ab134454)
      SDS-PAGE analysis of ab134454 at 0.5µg (lane 1) and 1.0 µg (lane 2).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab134454? Please let us know so that we can cite the reference in this datasheet.

    ab134454 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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