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I am using the Collagen I standard (Collagen I protein (ab7533) ) and noticed that anti-collagen I, anti-collagen III, and anti-collagen IV antibodies all detect it in a dot blot. Does this standard contain other types of collagen besides Collagen I?
Asked on Dec 16 2011
Our collagen I should not contain significant amounts of other types of collagen, however, the subunit strands of the different collagen types are similar and may share epitopes in common. If the antibodies used are not type specific this can cause reduced specificity. Our collagens are purified in a manner to select protein which has preserved 3D structure of type specific epitopes.
Answered on Dec 16 2011